Curcumin

Catalog No.S1848 Batch:S184806

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Technical Data

Formula

C21H20O6
Molecular Weight 368.38 CAS No. 458-37-7
Solubility (25°C)* In vitro DMSO 73 mg/mL (198.16 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Curcumin (Diferuloylmethane, Natural Yellow 3, Turmeric yellow) is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase（IC50~25 μM）and Histone deacetylase (HDAC); activates Nrf2 pathway and supresses the activation of NF-κB. Curcumin induces mitophagy, autophagy, apoptosis, and cell cycle arrest with antitumor activity. Curcumin reduces renal damage associated with rhabdomyolysis by decreasing ferroptosis-mediated cell death. Curcumin exhibits anti-infective properties against various human pathogens like the influenza virus, hepatitis C virus, HIV and so on.
Targets
Nrf2 [1]
(Cell-free assay)		 Ferroptosis [9] HDAC [7]
(Cell-free assay)		 NF-κB [8]
(Cell-free assay)		 p300 [6]
(Cell-free assay)
~25 μM
In vitro

Curcumin induces the expression of forkhead box protein O1 (FOXO1) through activation of extracellular signal-regulated kinase 1/2 signaling. Curcumin inhibits cell proliferation, which was associated with upregulation of the cyclin-dependent kinase inhibitors, p27 and p21, and downregulation of cyclin D1[2]. Curcumin induces endoplasmic reticulum (ER) stress and mitochondrial dysfunction as evidenced by up-regulation of CCAAT/enhancer binding protein homologous protein (CHOP), phosphorylation of JNK and down-regulation of SERCA2ATPase, release of cytochrome c, decrease of Bcl-2 and reduction of mitochondrial membrane potential in both AGS and HT-29 cells[3].
In vivo

Chronic treatment with curcumin significantly reverses the CMS-induced behavioral abnormalities in stressed rats. Additionally, curcumin effectively inhibits cytokine gene expression at both the mRNA and the protein level and reduces the activation of NF-κB[4].

Protocol (from reference)

Cell Assay:

[5]
  • Cell lines

    murine melanoma cell subline(B16-R)

  • Concentrations

    0-100 μM

  • Incubation Time

    24-48 h

  • Method

    1×104 B16-R cells are cultivated as monolayer culture for 12 hr. They were then incubated in 200 μL of RPMI, 10% FBS containing curcumin at final concentrations from 1–100 μM in 96-multiwell plates for 24-48 hr. After these incubations, cells are washed twice in PBS and 500 μl of fresh culture medium containing MTT (0.3 mg/mL) are added for colorimetric assay.
Animal Study:

[5]
  • Animal Models

    Female B6D2F1 mice (6-8 weeks old)

  • Dosages

    25 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Oncol Res, 2014, 21(3), 137-44]

Data from [Oncol Res, 2014, 21(3), 137-44]

Data from [Data independently produced by , , J Exp Clin Cancer Res, 2018, 37(1):303]

Data from [Data independently produced by , , Front Pharmacol, 2018, 9:60]

Selleck's Curcumin has been cited by 42 publications

The Cytotoxic Effect of Curcumin in Rhabdomyosarcoma Is Associated with the Modulation of AMPK, AKT/mTOR, STAT, and p53 Signaling [ Nutrients, 2023, 15(3)740] PubMed: 36771452
Curcumin suppress inflammatory response in traumatic brain injury via p38/MAPK signaling pathway [ Phytother Res, 2022, 36(3):1326-1337] PubMed: 35080289
MicroRNA profiling of paediatric AML with FLT-ITD or MLL-rearrangements: Expression signatures and in vitro modulation of miR-221-3p and miR-222-3p with BRD4/HATs inhibitors [ Oncol Rep, 2022, 48(6)221] PubMed: 36321792
Curcumin assists anti-EV71 activity of IFN-α by inhibiting IFNAR1 reduction in SH-SY5Y cells [ Gut Pathog, 2022, 14(1):8] PubMed: 35151347
Curcumin Inhibits the Growth and Metastasis of Melanoma via miR-222-3p/SOX10/Notch Axis [ Dis Markers, 2022, 2022:3129781] PubMed: 35585935
Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication [ Nat Metab, 2021, 10.1038/s42255-021-00479-4] PubMed: 34580494
Multifocal Organoid Capturing of Colon Cancer Reveals Pervasive Intratumoral Heterogenous Drug Responses [ Adv Sci (Weinh), 2021, e2103360] PubMed: 34918496
Low curcumin concentration enhances the anticancer effect of 5-fluorouracil against colorectal cancer [ Phytomedicine, 2021, 85:153547] PubMed: 33812170
Curcumin Blunts IL-6 Dependent Endothelial-to-Mesenchymal Transition to Alleviate Renal Allograft Fibrosis Through Autophagy Activation [ Front Immunol, 2021, 12:656242] PubMed: 34122411
Single-Oocyte Gene Expression Suggests That Curcumin Can Protect the Ovarian Reserve by Regulating the PTEN-AKT-FOXO3a Pathway [ Int J Mol Sci, 2021, 22(12)6570] PubMed: 34207376

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.