Prednisolone

Catalog No.S1737 Batch:S173704

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Technical Data

Formula

C21H28O5
Molecular Weight 360.44 CAS No. 50-24-8
Solubility (25°C)* In vitro DMSO 72 mg/mL (199.75 mM)
Ethanol 40 mg/mL (110.97 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5% DMSO 95% corn oil
4.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 20% PEG 300 5%Tween80 70% water
4.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 200 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue adding Adjust the volume to 1 mL with 700 μL ddH2O. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Prednisolone is a synthetic glucocorticoid with anti-inflammatory and immunomodulating properties.
Targets
Glucocorticoid receptor [1]
In vitro

Prednisolone (50 mg/kg, im) given 15 min before LPS-attenuated production of NO2- and NO3- by neutrophils and suppresses LPS-stimulated mRNA for NOS II in rat neutrophils. [1] Prednisolone reduces joint swelling through a mechanism associated with a reduction in IL-1beta and IL-6 protein and mRNA expression levels in SCW-induced arthritis rats. [2] Prednisolone's anti-inflammatory effects can be mediated by reducing cellular adhesion molecule (CAM) expression. Prednisolone (0.75 mg/kg) reduces macrophages (-59%, -57%), CD4(+) T-cells (-50%, -60%), CD8(+) T-cells (-58%, -48%), and eosinophils (-36%, -25%) in quadriceps and soleus muscles, respectively. Prednisolone-treated mice also exhibits decreased vascular P-selectin (-82%) and ICAM-1 (-52%) expression and fewer L-selectin (-79%) and ICAM-1 (-57%) expressing mononuclear cells in quadriceps. Prednisolone reduces sarcolemmal damage and degeneration as well in Dystrophin-deficient, mdx mice. [3]
In vivo

Prednisolone (5 mg/kg) causes alterations in diaphragmatic contractile properties and histological changes without fiber atrophy in rats. Prednisolone results in an increased number of diaphragmatic bundles in rats. [4] Prednisolone induces a significant decline of PL-pO2, while CoBF, CMs, CAPs, and ABRs revealed no change in guinea pigs. [5]

Protocol (from reference)

Customer Product Validation

, , PLoS One, 2014, 9(12):e111840.

Data from [Data independently produced by , , Leuk Lymphoma, 2014, 55(5):1144-50. ]

Selleck's Prednisolone has been cited by 16 publications

PRMT5 triggers glucocorticoid-induced cell migration in triple-negative breast cancer [ Life Sci Alliance, 2023, 6(10)e202302009] PubMed: 37536978
PRMT5 triggers glucocorticoid-induced cell migration in triple-negative breast cancer [ Life Sci Alliance, 2023, 6(10)e202302009] PubMed: 37536978
Reversal of IKZF1-induced glucocorticoid resistance by dual targeting of AKT and ERK signaling pathways [ Front Oncol, 2022, 12:905665] PubMed: 36119546
Application of a newly-developed cynomolgus macaque BiTE-mediated cytotoxic T-lymphocyte activity assay to various immunomodulatory agents in vitro [ J Immunotoxicol, 2021, 18(1):154-162] PubMed: 34714999
In vitro effect of biological and conventional disease-modifying antirheumatic drugs on fibrocyte differentiation in patients with rheumatoid arthritis and healthy controls [ Eur J Rheumatol, 2021, 10.5152/eurjrheum.2021.20054] PubMed: 34059186
Glucocorticoids improve severe or critical COVID-19 by activating ACE2 and reducing IL-6 levels [ Int J Biol Sci, 2020, 16(13):2382-2391] PubMed: 32760206
WEE1 inhibition synergizes with CHOP chemotherapy and radiation therapy through induction of premature mitotic entry and DNA damage in diffuse large B-cell lymphoma [ Ther Adv Hematol, 2020, 11:2040620719898373] PubMed: 32010435
WEE1 inhibition synergizes with CHOP chemotherapy and radiation therapy through induction of premature mitotic entry and DNA damage in diffuse large B-cell lymphoma. [ Ther Adv Hematol, 2020, 11:2040620719898373] PubMed: 32010435
High-throughput liquid chromatography tandem mass spectrometry assay as initial testing procedure for analysis of total urinary fraction [ Drug Test Anal, 2020, 10.1002/dta.2917] PubMed: 32852861
A curative combination cancer therapy achieves high fractional cell killing through low cross-resistance and drug additivity. [ Elife, 2019, 8] PubMed: 31742555

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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