ABTL-0812

Catalog No.S9611 Batch:S961101

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Technical Data

Formula

C18H32O3
Molecular Weight 296.44 CAS No. 57818-44-7
Solubility (25°C)* In vitro  
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description ABTL0812 (α-Hydroxylinoleic acid, LP-10218, SCLN-0812) inhibits Akt/mTOR axis by inducing the overexpression of TRIB3 and activating autophagy in lung squamous carcinoma cell lines. ABTL0812 also induces AMPK activation and ROS accumulation.
Targets
Akt [1] mTOR [1]
In vitro

ABTL0812 inhibits Akt/mTOR axis by inducing the overexpression of TRIB3 and activating autophagy in lung squamous carcinoma cell lines. Furthermore, treatment with ABTL0812 also induces AMPK activation and ROS accumulation. Moreover, combination of ABTL0812 with chemotherapy markedly increases the therapeutic effect of chemotherapy without increasing toxicity. Combination of ABTL0812 and chemotherapy induces nonapoptotic cell death mediated by TRIB3 activation and autophagy induction.[1]
In vivo

ABTL0812 enhances carboplatin and paclitaxel antitumor activity both in adenocarcinomas and squamous lung xenografts.[1]
Density 1.0 g/mL

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    Human adenocarcinoma cell lines (A549, NCI-H1975), squamous-cell carcinoma (NCI-H520, NCI-H157)

  • Concentrations

    0–200 μM

  • Incubation Time

    72 h

  • Method

    Briefly, for docetaxel studies, subconfluent cultures of adenocarcinoma lung cancer cells are incubated for 72 hr in the presence of an increasing concentration of ABTL0812 (0–200 μM), docetaxel (0–100 μM) or the combination of increasing doses of docetaxel with ABTL0812 20 μM. For paclitaxel studies, subconfluent cultures of adenocarcinoma and squamous lung cancer cell lines are incubated for 48 hr in the presence of an increasing concentration of paclitaxel (0–1 μM) or the combination of increasing doses of paclitaxel with sub-IC50 concentrations of ABTL0812 (5–30 μM).
Animal Study:

[1]
  • Animal Models

    5-week-old female athymic nude Foxn 1nu nu/nu mice

  • Dosages

    30 mg/kg, 120 mg/kg

  • Administration

    Oral gavage

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.