Adalimumab (anti-TNF-alpha)

Catalog No.A2010        Batch: A201002

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Technical Data

CAS No. 331731-18-1
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 98%
Protein concentration 5.00mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Adalimumab (anti-TNF-alpha) is the first fully human, recombinant IgG1 monoclonal antibody that specifically targets human TNF-alpha, MW: 144.19 KD.
Targets
TNF-α [1]
In vitro

Adalimumab treatment alters the secretion of cytokines.[1]
In vivo

We injected adalimumab intraductally into the MG of adult mice;this was found to stimulate BSC multipotency.[2]

Protocol (Only for Reference)

Cell Assay:

  • Fa2N-4 cells were co-cultivated (CC) with monocyte derived macrophages (MDMs) for 24 h. Physiological (15%) and pro-inflammatory (50%) MDM-to-Fa2N-4 ratios were used. All measured cytokine concentrations were related to the corresponding control (fold change) to analyse (A) the IL-8 neutralising antibody (αIL-8, 100 ng/ml) specific effect and (B) the TNF-α neutralising effect of Adalimumab.
Animal Study:

  • Animal Models: K5CreER, K8rtTA, TetO-DTA mice
    Dosages: 0.15 mg
    Administration: i.d.
    Reference:[2]

Selleck's Adalimumab (anti-TNF-alpha) has been cited by 12 publications

Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
HS 3D-SeboSkin Model Enables the Preclinical Exploration of Therapeutic Candidates for Hidradenitis Suppurativa/Acne Inversa [ Pharmaceutics, 2023, 15(2)619] PubMed: 36839941
Identification of hub necroptosis-related lncRNAs for prognosis prediction of esophageal carcinoma [ Aging (Albany NY), 2023, 15(11):4794-4819] PubMed: 37263709
Characterization and optimization of variability in a human colonic epithelium culture model [ bioRxiv, 2023, 2023.09.22.559007] PubMed: 37790345
Characterization and optimization of variability in a human colonic epithelium culture model [ bioRxiv, 2023, 2023.09.22.559007] PubMed: 37790345
Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities [ Mol Ther, 2022, S1525-0016(22)00418-X] PubMed: 35791880
Novel indirect co-culture of immortalised hepatocytes with monocyte derived macrophages is characterised by pro-inflammatory cytokine networks [ Toxicol In Vitro, 2021, 73:105134] PubMed: 33662514
Protective Effect of Adalimumab on Diabetic Nephropathy by Regulating TNF-α Signal Pathway [ Iran J Public Health, 2021, 50(12):2536-2545] PubMed: 36317034
Heterotypic cell-cell communication regulates glandular stem cell multipotency [ Nature, 2020, 584(7822):608-613] PubMed: 32848220
Heterotypic cell-cell communication regulates glandular stem cell multipotency [ Nature, 2020, 584(7822):608-613] PubMed: 32848220

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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