ATP

Catalog No.S5260 Batch:S526001

Technical Data

Formula	C₁₀H₁₆N₅O₁₃P₃
Molecular Weight	507.18	CAS No.	56-65-5
Solubility (25°C)*	In vitro	Water	100 mg/mL (197.16 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	ATP (Adenosine-Triphosphate, Adenosine 5'-triphosphate) is a multifunctional nucleoside triphosphate and an important endogenous signaling molecule in immunity and inflammation.
In vitro	ATP, a multifunctional nucleoside triphosphate and an important endogenous signaling molecule, restores signaling responses to anti-TCR treatment in Jurkat cells.^[2]
In vivo	ATP can activate the NLRP3 inflammasome and enhance the production of pro-inflammatory cytokines, thus protecting mice against bacterial infection.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Jurkat cells Concentrations 50 mM Incubation Time -- Method Cells are washed with calcium-free and magnesium-free 1×DPBS to remove calcium and magnesium. Then cells are suspended with 450 µL 1×DPBS into a 1.5 mL centrifugal tube, treated with 50 mL Streptolysin O (0.5 µg/ml), and incubated 60 mins at 37℃. Next, cells are centrifuged at 400 g for 4 mins to discard the supernatant. Cells are re-suspended with 100 mL 1×DPBS, treated with 11 µL 50 mM ATP, and incubated in the 37℃ for indicated time.
Animal Study:^{^[3]}	Animal Models Kunming mice Dosages 50 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
Jurkat cells
Concentrations
50 mM
Incubation Time
--
Method
Cells are washed with calcium-free and magnesium-free 1×DPBS to remove calcium and magnesium. Then cells are suspended with 450 µL 1×DPBS into a 1.5 mL centrifugal tube, treated with 50 mL Streptolysin O (0.5 µg/ml), and incubated 60 mins at 37℃. Next, cells are centrifuged at 400 g for 4 mins to discard the supernatant. Cells are re-suspended with 100 mL 1×DPBS, treated with 11 µL 50 mM ATP, and incubated in the 37℃ for indicated time.

Animal Study:^{^[3]}

Animal Models
Kunming mice
Dosages
50 mg/kg
Administration
i.p.

References

Selleck's ATP has been cited by 6 publications

The ARID1A-METTL3-m6A axis ensures effective RNase H1-mediated resolution of R-loops and genome stability [ Cell Rep, 2024, 43(2):113779]	PubMed: 38358891
Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing [ Cell Rep Med, 2023, 4(8):101129]	PubMed: 37480849
CD39 pathway inhibits Th1 cell function in tuberculosis [ Immunology, 2022, 10.1111/imm.13493]	PubMed: 35574713
ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury [ Cell Signal, 2022, 93:110304]	PubMed: 35278669
NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells [ Cell Rep, 2021, 36(6):109516]	PubMed: 34380043
Necroptosis Underlies Neutrophilic Inflammation Associated with the Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) [ J Inflamm Res, 2021, 14:3969-3983]	PubMed: 34429629

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.