Blasticidin S HCl

Catalog No.S7419 Batch:S741910

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Technical Data

Formula

C17H27ClN8O5
Molecular Weight 458.9 CAS No. 3513-03-9
Solubility (25°C)* In vitro Water 46 mg/mL (100.23 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%H2O 40%PEG 300 5%Tween 80 50%H2O
4.6mg/ml Taking the 1 mL working solution as an example, add 50 μL 92 mg/mL clarified H2O stock solution to 400 μL PEG300, mix evenly to clarify; add 50 μL Tween-80 to the above system, mix evenly to clarify; Then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes.
Targets
protein synthesis [1] DNA synthesis [1]
In vitro

Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1]

Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2]

Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3]

Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4]
In vivo

Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes.

Protocol (from reference)

Cell Assay:

[6]
  • Cell lines

    HEK293T cells

  • Concentrations

    5 µg/ml

  • Incubation Time

    7 days

  • Method

    Lentivirus infected cells were maintained in 5 μg/ml blasticidin containing culture medium for 7d, with fresh medium exchange every day.

Selleck's Blasticidin S HCl has been cited by 23 publications

Chicoric Acid Ameliorated Beta-Amyloid Pathology and Enhanced Expression of Synaptic-Function-Related Markers via L1CAM in Alzheimer's Disease Models [ Int J Mol Sci, 2024, 25(6)3408] PubMed: 38542381
Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51 [ Nat Commun, 2023, 10.1038/s41467-023-42850-x] PubMed: 37973845
Genetically encoded photocatalytic protein labeling enables spatially-resolved profiling of intracellular proteome [ Nat Commun, 2023, 14(1):2978] PubMed: 37221179
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin [ Mol Cell, 2023, 83(13):2206-2221.e11] PubMed: 37311463
Targeting VCP potentiates immune checkpoint therapy for colorectal cancer [ Cell Rep, 2023, 42(11):113318] PubMed: 37865914
MERR APEX-seq protocol for profiling the subcellular nascent transcriptome in mammalian cells [ STAR Protoc, 2023, 4(1):102057] PubMed: 36853684
Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators [ Cell Rep Methods, 2023, 3(8):100544] PubMed: 37671014
A Nodal enhanced micropeptide NEMEP regulates glucose uptake during mesendoderm differentiation of embryonic stem cells [ Nat Commun, 2022, 13(1):3984] PubMed: 35810171

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.