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Technical Data
| Formula | C20H30N2O4 |
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| Molecular Weight | 362.46 | CAS No. | 117591-20-5 | |
| Solubility (25°C)* | In vitro | DMSO | 72 mg/mL (198.64 mM) | |
| Ethanol | 72 mg/mL (198.64 mM) | |||
| Water | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Preparing Stock Solutions
Biological Activity
| Description | Calpeptin is a potent, cell-permeable calpain inhibitor with ID50 of 52 nM, 34 nM, 138 nM, and 40 nM for Calpain I (porcine erythrocytes), Calpain II (porcine kidney), Papainb, and Calpain I (human platelets), respectively. Calpeptin attenuates apoptosis and intracellular inflammatory changes in muscle cells. | ||||||||
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| Targets |
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| In vitro | Calpeptin inhibits 20K phosphorylation in a dose-related manner in platelets stimulated by thrombin, ionomycin or collagen. [1] In differentiating PC12 cells, Calpeptin promotes neurite elongation via inhibition of Calpain activity. [2] In rat retinal ganglion cells, Calpeptin attenuates apoptosis, maintains normal whole-cell membrane potential, and thus provides functional neuroprotection. [3] | ||||||||
| In vivo | In a feline right ventricular (RV) PO (RVPO) model, calpeptin (0.6 mg/kg, i.v.) blocks the activation of calpain and caspase-3, cleavage of their substrates, and cardiomyocyte programmed cell death. [4] In a rat focal cerebral ischemia–reperfusion injury model, Calpeptin reduces the neuronal apoptosis in hippocampal CA1 sector via inhibition of the expression of Caspase-3. [5] |
Protocol (from reference)
| Animal Study:[4] |
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References
Customer Product Validation
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Data from [Data independently produced by , , Oncol Rep, 2017, 37(6):3651-3659]
Selleck's Calpeptin has been cited by 25 publications
| Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling [ Proc Natl Acad Sci U S A, 2023, 120(43):e2304288120] | PubMed: 37844244 |
| Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling [ Proc Natl Acad Sci U S A, 2023, 120(43):e2304288120] | PubMed: 37844244 |
| The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential [ EMBO Rep, 2023, e55069.] | PubMed: 36744297 |
| The lactate-NAD+ axis activates cancer-associated fibroblasts by downregulating p62 [ Cell Rep, 2022, 39(6):110792] | PubMed: 35545049 |
| Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis [ Oxid Med Cell Longev, 2022, 2022:2566917] | PubMed: 35498131 |
| Calpain-1: a Novel Antiviral Host Factor Identified in Porcine Small Intestinal Mucus [ mBio, 2022, 13(5):e0035822] | PubMed: 36102516 |
| Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy [ Comput Struct Biotechnol J, 2022, 20:2442-2454] | PubMed: 35602976 |
| Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis [ Cell Death Discov, 2021, 7(1):374] | PubMed: 34864819 |
| Xanthohumol Is a Potent Pan-Inhibitor of Coronaviruses Targeting Main Protease [ Int J Mol Sci, 2021, 22(22)12134] | PubMed: 34830015 |
| Caspase-mediated cleavage of miRNA processing proteins Drosha, DGCR8, Dicer, and TRBP2 in heat-shocked cells and its inhibition by HSP70 overexpression [ Cell Stress Chaperones, 2021, 10.1007/s12192-021-01242-0] | PubMed: 34719748 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.