Capsaicin(Vanilloid)

Catalog No.S1990 Batch:S199002

Technical Data

Formula	C₁₈H₂₇NO₃
Molecular Weight	305.41	CAS No.	404-86-4
Solubility (25°C)*	In vitro	DMSO	61 mg/mL (199.73 mM)
		Ethanol	61 mg/mL (199.73 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Capsaicin(Vanilloid) is an active component of chili peppers, which are plants belonging to the genus Capsicum.Capsaici is a TRPV1 agonist with an EC50 of 0.29 μM in HEK293 cells.
In vitro	Capsaicin, a TRPV-1 agonist, dose-dependently inhibits the proliferation of the HCC cell lines PLC/PRF/7, HuH7 and HepG2, which is not effected by TRPV1 channel blockers. Combining capsaicin and sorafenib significantly enhances the suppression on cell proliferation, achieving a high-level synergistic effect and promoting HCC cell apoptosis through the enhancement of p-ERK and reduction of p-STAT3 signaling.^[1]
In vivo	Combining capsaicin and sorafenib enhances inhibition of xenograft tumor growth in nude mice, with no apparent gross toxicity.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HCC cells PLC/PRF/5, HuH7, and HepG2 Concentrations 50, 100, 150, 200 and 250 μM Incubation Time 72 h Method Cells (5×10³ per well) seeded in 96-well plates are exposed to capsaicin, sorafenib, or their combination for 72 h. Then, the medium is removed and the wells are washed with phosphate-buffered saline (PBS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is performed by adding 20 μL of MTT to the cells for 4 h. Light absorbance is measured.

Cell Assay:^{^[1]}

Cell lines
HCC cells PLC/PRF/5, HuH7, and HepG2
Concentrations
50, 100, 150, 200 and 250 μM
Incubation Time
72 h
Method
Cells (5×10³ per well) seeded in 96-well plates are exposed to capsaicin, sorafenib, or their combination for 72 h. Then, the medium is removed and the wells are washed with phosphate-buffered saline (PBS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is performed by adding 20 μL of MTT to the cells for 4 h. Light absorbance is measured.

References

[1] Zhang SS, et al. Acta Pharmacol Sin. 2018;39(3):438-448.

Selleck's Capsaicin(Vanilloid) has been cited by 10 publications

Phytoformulation with hydroxycitric acid and capsaicin protects against high-fat-diet-induced obesity cardiomyopathy by reducing cardiac lipid deposition and ameliorating inflammation and apoptosis in the heart [ J Tradit Complement Med, 2024, 14(2):162-172]	PubMed: 38481548
Effects of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins on Hepatocellular Carcinoma Cells [ Int J Mol Sci, 2023, 24(4)3823]	PubMed: 36835232
Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer's Disease [ Oxid Med Cell Longev, 2022, 2022:8096009]	PubMed: 35116093
Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells [ Mar Drugs, 2022, 20(6)378]	PubMed: 35736181
Electroacupuncture Pretreatment Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through Transient Receptor Potential Vanilloid 1-Mediated Anti-apoptosis via Inhibiting NF-κB Signaling Pathway [ Neuroscience, 2021, S0306-4522(21)00641-2]	PubMed: 34929338
Paeoniflorin relieves LPS-induced inflammatory pain in mice by inhibiting NLRP3 inflammasome activation via transient receptor potential vanilloid 1. [ J Leukoc Biol, 2020, 10.1002/JLB.3MA0220-355R]	PubMed: 32083340
Electroacupuncture Pretreatment Elicits Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Rats Associated with Transient Receptor Potential Vanilloid 1-Mediated Anti-Oxidant Stress and Anti-Inflammation [ Inflammation, 2019, 10.1007/s10753-019-01040-y]	PubMed: 31190106
Capsaicin enhances the antitumor activity of sorafenib in hepatocellular carcinoma cells and mouse xenograft tumors through increased ERK signaling [ Acta Pharmacol Sin, 2018, 39(3):438-448]	PubMed: 29188798
Capsaicin and sorafenib combination treatment exerts synergistic anti‑hepatocellular carcinoma activity by suppressing EGFR and PI3K/Akt/mTOR signaling [ Oncol Rep, 2018, 40(6):3235-3248]	PubMed: 30272354
Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor. [ J Diabetes Complications, 2015, 29(3):323-8]	PubMed: 25650182

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SHIPPING AND STORAGE
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