Capsaicin(Vanilloid)

Catalog No.S1990 Batch:S199003

Technical Data

Formula

C₁₈H₂₇NO₃

Molecular Weight

305.41

CAS No.

404-86-4

Solubility (25°C)*

In vitro

DMSO

54 mg/mL (176.81 mM)

Ethanol

54 mg/mL (176.81 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution

5%DMSO 1 Corn oil

2.7mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Capsaicin(Vanilloid) is an active component of chili peppers, which are plants belonging to the genus Capsicum.Capsaici is a TRPV1 agonist with an EC50 of 0.29 μM in HEK293 cells.
In vitro	Capsaicin, a TRPV-1 agonist, dose-dependently inhibits the proliferation of the HCC cell lines PLC/PRF/7, HuH7 and HepG2, which is not effected by TRPV1 channel blockers. Combining capsaicin and sorafenib significantly enhances the suppression on cell proliferation, achieving a high-level synergistic effect and promoting HCC cell apoptosis through the enhancement of p-ERK and reduction of p-STAT3 signaling.^[1]
In vivo	Combining capsaicin and sorafenib enhances inhibition of xenograft tumor growth in nude mice, with no apparent gross toxicity.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HCC cells PLC/PRF/5, HuH7, and HepG2 Concentrations 50, 100, 150, 200 and 250 μM Incubation Time 72 h Method Cells (5×10³ per well) seeded in 96-well plates are exposed to capsaicin, sorafenib, or their combination for 72 h. Then, the medium is removed and the wells are washed with phosphate-buffered saline (PBS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is performed by adding 20 μL of MTT to the cells for 4 h. Light absorbance is measured.

Cell Assay:^{^[1]}

Cell lines
HCC cells PLC/PRF/5, HuH7, and HepG2
Concentrations
50, 100, 150, 200 and 250 μM
Incubation Time
72 h
Method
Cells (5×10³ per well) seeded in 96-well plates are exposed to capsaicin, sorafenib, or their combination for 72 h. Then, the medium is removed and the wells are washed with phosphate-buffered saline (PBS). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is performed by adding 20 μL of MTT to the cells for 4 h. Light absorbance is measured.

References

[1] Zhang SS, et al. Acta Pharmacol Sin. 2018;39(3):438-448.

Selleck's Capsaicin(Vanilloid) has been cited by 10 publications

Phytoformulation with hydroxycitric acid and capsaicin protects against high-fat-diet-induced obesity cardiomyopathy by reducing cardiac lipid deposition and ameliorating inflammation and apoptosis in the heart [ J Tradit Complement Med, 2024, 14(2):162-172]	PubMed: 38481548
Effects of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins on Hepatocellular Carcinoma Cells [ Int J Mol Sci, 2023, 24(4)3823]	PubMed: 36835232
Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer's Disease [ Oxid Med Cell Longev, 2022, 2022:8096009]	PubMed: 35116093
Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells [ Mar Drugs, 2022, 20(6)378]	PubMed: 35736181
Electroacupuncture Pretreatment Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through Transient Receptor Potential Vanilloid 1-Mediated Anti-apoptosis via Inhibiting NF-κB Signaling Pathway [ Neuroscience, 2021, S0306-4522(21)00641-2]	PubMed: 34929338
Paeoniflorin relieves LPS-induced inflammatory pain in mice by inhibiting NLRP3 inflammasome activation via transient receptor potential vanilloid 1. [ J Leukoc Biol, 2020, 10.1002/JLB.3MA0220-355R]	PubMed: 32083340
Electroacupuncture Pretreatment Elicits Neuroprotection Against Cerebral Ischemia-Reperfusion Injury in Rats Associated with Transient Receptor Potential Vanilloid 1-Mediated Anti-Oxidant Stress and Anti-Inflammation [ Inflammation, 2019, 10.1007/s10753-019-01040-y]	PubMed: 31190106
Capsaicin enhances the antitumor activity of sorafenib in hepatocellular carcinoma cells and mouse xenograft tumors through increased ERK signaling [ Acta Pharmacol Sin, 2018, 39(3):438-448]	PubMed: 29188798
Capsaicin and sorafenib combination treatment exerts synergistic anti‑hepatocellular carcinoma activity by suppressing EGFR and PI3K/Akt/mTOR signaling [ Oncol Rep, 2018, 40(6):3235-3248]	PubMed: 30272354
Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor. [ J Diabetes Complications, 2015, 29(3):323-8]	PubMed: 25650182

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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