CNO (Clozapine N-oxide)

Catalog No.S6887 Batch:S688703

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Technical Data

Formula

C18H19ClN4O
Molecular Weight 342.82 CAS No. 34233-69-7
Solubility (25°C)* In vitro DMSO 69 mg/mL (201.27 mM)
Water 10 mg/mL (29.16 mM)
Ethanol 3 mg/mL (8.75 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description CNO (Clozapine N-oxide) is a metabolite of Clozapine (GLXC-06516) and an agonist of human muscarinic designer receptors (Designer Receptors Exclusively Activated by Designer Drugs, DREADDs). Clozapine is a potent dopamine (DA) antagonist and a selective muscarinic M4 receptor agonist.Solutions are unstable and should be fresh-prepared.
Targets
DREADD [1] Dopamine receptor [2] muscarinic M4 receptor [1]
In vitro

CNO is a synthetic small molecule actuator for muscarinic acetylcholine DREADDs.
In vivo

Clozapine N-oxide (CNO), an agonist of human muscarinic designer receptor (DREADDs), is converted to clozapine and exerts clozapine-like behavioral effects in both mice and rats further emphasizes the need for appropriate control groups in studies employing DREADDs.[4]

Protocol (from reference)

Animal Study:

[4]
  • Animal Models

    standard laboratory rats and mice

  • Dosages

    1.0, 3.2, 10.0 mg/kg for rats; 1.25, 2.5, 5.0, 10.0, 20.0 mg/kg for mice

  • Administration

    i.p.

Selleck's CNO (Clozapine N-oxide) has been cited by 6 publications

Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion [ Nat Commun, 2023, 10.1038/s41467-023-42814-1] PubMed: 37903803
Nucleus accumbens circuit disinhibits lateral hypothalamus glutamatergic neurons contributing to morphine withdrawal memory in male mice [ Nat Commun, 2023, 14(1):71] PubMed: 36604429
Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion [ Nat Commun, 2023, 14(1):6923] PubMed: 37903803
Tet2 acts in the lateral habenula to regulate social preference in mice [ Cell Rep, 2023, 42(7):112695] PubMed: 37402169
Excitatory neurons in the lateral parabrachial nucleus mediate the interruptive effect of inflammatory pain on a sustained attention task [ J Transl Med, 2023, 21(1):896] PubMed: 38072957
Activation of astrocyte Gq pathway in hippocampal CA1 region attenuates anesthesia/surgery induced cognitive dysfunction in aged mice [ Front Aging Neurosci, 2022, 14:1040569] PubMed: 36437995

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.