Dimercaprol

Catalog No.S4446 Batch:S444601

Technical Data

Formula	C₃H₈OS₂
Molecular Weight	124.22	CAS No.	59-52-9
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

Dimercaprol (Bal, 2,3-Dimercapto-1-propanol, Dithioglycerol) is an acrolein scavenger that inhibits HIV-1 tat activity, viral production, and infectivity in vitro.

Targets

HIV-1 tat ^[1]

In vitro

Dimercaprol exerts its acrolein scavenging capability, significantly protects PC-12 cells from acrolein-mediated cell death in a dose dependent manner.^[3]

In vivo

Dimercaprol could significantly reduce acrolein contents in spinal cord tissue following a spinal cord contusion injury in rats.^[3]

Density

1.239 g/mL

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines PC-12 cells Concentrations 10, 25, 50 and 100 μM Incubation Time 4 h Method Cell viability was tested by WST-1 ([2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium]) assay. Both dimercaprol and acrolein were dissolved in Hank’s Balanced Salt Solution (HBSS). The final concentration of acrolein was 100 μM. The final concentrations of dimercaprol were 10, 25, 50 and 100 μM. PC-12 cells were seeded in 12-well plates at the concentration of 10⁵ cells/well. After overnight incubation, acrolein was added into each well of acrolein group and treatment groups. Equal volumes of HBSS were added into wells of the negative control group. After incubation for 15 minutes, dimercaprol was added at the desired final concentrations (10, 25, 50 and 100 μM) into wells of treatment groups and equal volume of HBSS was added into other wells. The initial 15-minute incubation in acrolein was designed to mimic pathological conditions by allowing acrolein to enter the cells. WST-1 assay solution was added into every well after incubating 4 h, and then allowed to incubate for an additional 2 h.
Animal Study:^{^[3]}	Animal Models Male Sprague–Dawley rats of spinal cord contusion injury model Dosages 5 mg/kg Administration i.p

Cell Assay:^{^[3]}

Cell lines
PC-12 cells
Concentrations
10, 25, 50 and 100 μM
Incubation Time
4 h
Method
Cell viability was tested by WST-1 ([2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium]) assay. Both dimercaprol and acrolein were dissolved in Hank’s Balanced Salt Solution (HBSS). The final concentration of acrolein was 100 μM. The final concentrations of dimercaprol were 10, 25, 50 and 100 μM. PC-12 cells were seeded in 12-well plates at the concentration of 10⁵ cells/well. After overnight incubation, acrolein was added into each well of acrolein group and treatment groups. Equal volumes of HBSS were added into wells of the negative control group. After incubation for 15 minutes, dimercaprol was added at the desired final concentrations (10, 25, 50 and 100 μM) into wells of treatment groups and equal volume of HBSS was added into other wells. The initial 15-minute incubation in acrolein was designed to mimic pathological conditions by allowing acrolein to enter the cells. WST-1 assay solution was added into every well after incubating 4 h, and then allowed to incubate for an additional 2 h.

Animal Study:^{^[3]}

Animal Models
Male Sprague–Dawley rats of spinal cord contusion injury model
Dosages
5 mg/kg
Administration
i.p

References

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SHIPPING AND STORAGE
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