Doxycycline

Catalog No.S5159 Batch:S515901

Technical Data

Formula	C₂₂H₂₄N₂O₈
Molecular Weight	444.43	CAS No.	564-25-0
Solubility (25°C)*	In vitro	DMSO	88 mg/mL (198.0 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.
In vitro	100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used^[1]. Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes^[2].
In vivo	Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)^[2].

Description

Doxycycline (Vibramycin, Doxytetracycline, Doxiciclina, Doxycyclinum) is an antibiotic that is used in the treatment of a number of types of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor.

In vitro

100 ng/mL-5 µg/mL Dox can significantly alter the metabolic profile of the cell, as well as reduce the proliferative rate, though the effect size depends upon the particular cell line used^[1].

Doxycycline arrests cells in G1-S phase of the cell cycle in PANC-1 cells and induces the expression of p53 and its downstream target p21. It down-regulates antiapoptotic genes and induces proapoptotic genes. Doxycycline also induces apoptosis through a Fas/Fas-ligand dependent pathway in Jurkat T lymphocytes^[2].

In vivo

Doxycycline successfully reduces tumor growth in vivo (inhibited pancreatic cancer cell growth)^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines Concentrations 100 ng/mL, 1 µg/mL Incubation Time 24, 72, and 96 hours Method Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.
Animal Study:^{^[2]}	Animal Models Male nu/nu mice Dosages 35 mg/pellet Administration s.c.

Cell Assay:^{^[1]}

Cell lines
U87 astrocytoma cells, lung cancer cell lines H157 and H1437, LNCaP prostate cancer and HeLa cervical cancer cell lines
Concentrations
100 ng/mL, 1 µg/mL
Incubation Time
24, 72, and 96 hours
Method
Cells are seeded in 6 well plates at 50,000 cells per well and treated with Dox (100 ng/mL, 1 µg/mL, or vehicle control). Cells are counted 24, 72, and 96 hours after plating using a particle counter.

Animal Study:^{^[2]}

Animal Models
Male nu/nu mice
Dosages
35 mg/pellet
Administration
s.c.

References

Selleck's Doxycycline has been cited by 37 publications

Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human [ Nat Genet, 2024, 56(1):124-135]	PubMed: 38195860
UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis [ Cell Rep, 2024, 43(2):113808]	PubMed: 38367236
The Lin28b/Wnt5a axis drives pancreas cancer through crosstalk between cancer associated fibroblasts and tumor epithelium [ Nat Commun, 2023, 14(1):6885]	PubMed: 37898598
IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma [ Nat Commun, 2023, 14(1):6781]	PubMed: 37880243
IDH1 mutation impairs antiviral response and potentiates oncolytic virotherapy in glioma [ Nat Commun, 2023, 10.1038/s41467-023-42545-3]	PubMed: 37880243
DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination [ Adv Sci (Weinh), 2023, 10(14):e2204438]	PubMed: 36965071
A kinome-wide CRISPR screen identifies CK1α as a target to overcome enzalutamide resistance of prostate cancer [ Cell Rep Med, 2023, 4(4):101015]	PubMed: 37075701
Targeting VCP potentiates immune checkpoint therapy for colorectal cancer [ Cell Rep, 2023, 42(11):113318]	PubMed: 37865914
NF-κB represses retinoic acid receptor-mediated GPRC5A transactivation in lung epithelial cells to promote neoplasia [ JCI Insight, 2023, 8(1)e153976]	PubMed: 36413416
APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress [ J Transl Med, 2023, 21(1):183]	PubMed: 36894994

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.