Durvalumab (anti-PD-L1)

Catalog No.A2013        Batch: A201304

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Technical Data

CAS No. 1428935-60-7
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99.68%
Protein concentration 5 mg/ml
Endotoxin Level <1 EU/mg

Biological Activity

Description Durvalumab (anti-PD-L1) is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.
Targets
PD-L1/CD80 [1]
(HTRF assay)		 PD-1/PD-L1 interaction [1]
(HTRF assay)
0.04 nM 0.1 nM
In vitro

The labeled radioligand shows good affinity to high PD-L1 expression cells and could be blocked with excess unlabeled intact durvalumab.[1]
In vivo

The peak tumor uptake of 124I-Durva-F(ab’)2 was close to 124I-Durva, but much earlier (5.29±0.42% ID/g for 124I-Durva-F(ab’)2 at 12 h vs 5.18±0.73% ID/g for 124I-Durva at 48 h). Compared with 124I-Durva, an accelerated blood clearance was observed for 124I-Durva-F(ab’)2, allowing for a higher tumor-to-background ratio.[1]

Protocol (Only for Reference)

Cell Assay:

  • The A549 and H460 cells were cultured in six-well plates (~106 cells/well) and divided into two groups. All of the wells were incubated with 5% BSA for blocking nonspecific binding. Group A was presaturated with 100-fold excess of unlabeled durvalumab to block the binding of 124I-Durva-F(ab′)2, and group B had only the medium added. After 30 min, 2 nM of 124I-Durva-F(ab′)2 was added to each group and incubated for 1 h at 37 °C in a cell incubator. After supernatant collection, the cells were lysed using 2 M of NaOH.
Animal Study:

  • Animal Models: H460 and A549 tumor-bearing mice
    Dosages: 20 μCi
    Administration: i.v.

Customer Product Validation

Selleck's Durvalumab (anti-PD-L1) has been cited by 14 publications

Noninvasive PET imaging of tumor PD-L1 expression with 64Cu-labeled Durvalumab [ Am J Nucl Med Mol Imaging, 2024, 14(1):31-40] PubMed: 38500749
Development of a highly sensitive chemiluminescence immunoassay using a novel signal-enhanced detection system for quantitation of durvalumab, an immune-checkpoint inhibitor monoclonal antibody used for immunotherapy of lung cancer [ Heliyon, 2023, 9(6):e15782] PubMed: 37389074
Development of a highly sensitive chemiluminescence immunoassay using a novel signal-enhanced detection system for quantitation of durvalumab, an immune [ Heliyon, 2023, 9(6):e15782.] PubMed: 37389074
Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor [ Cancer Discov, 2022, 12(6):1482-1499] PubMed: 35254416
Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor [ Cancer Discov, 2022, 12(6):1482-1499] PubMed: 35254416
SBSN drives bladder cancer metastasis via EGFR/SRC/STAT3 signalling [ J Med Chem, 2022, 65(5):3879-3893] PubMed: 35188766
Sunitinib potentiates the cytotoxic effect of electrochemotherapy in pancreatic carcinoma cells [ Mol Pharm, 2022, 10.1021/acs.molpharmaceut.2c00084] PubMed: 35244407
Programmed death ligand-1 regulates angiogenesis and metastasis by participating in the c-JUN/VEGFR2 signaling axis in ovarian cancer [ Cancer Commun (Lond), 2021, 41(6):511-527] PubMed: 33939321
Novel Biphenyl Pyridines as Potent Small-Molecule Inhibitors Targeting the Programmed Cell Death-1/Programmed Cell Death-Ligand 1 Interaction [ J Med Chem, 2021, 64(11):7390-7403] PubMed: 34056906
Galvanotactic Migration of Glioblastoma and Brain Metastases Cells [ J Med Chem, 2021, 64(15):11614-11636] PubMed: 34313116

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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