Erastin

Catalog No.S7242 Batch:S724204

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Technical Data

Formula

C30H31ClN4O4
Molecular Weight 547.04 CAS No. 571203-78-6
Solubility (25°C)* In vitro DMSO (warmed with 50ºC water bath) 25 mg/mL (45.7 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
10%DMSO 40%PEG300 5%Tween80 45%ddH2O
1.67mg/ml Taking the 1 mL working solution as an example, add 100 μL of 16.67 mg/ml clear DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 450 μL of ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Erastin is a ferroptosis activator by acting on mitochondrial VDAC, exhibiting selectivity for tumor cells bearing oncogenic RAS. Solutions are unstable and should be fresh-prepared.
Targets
Ferroptosis [1]
In vitro

Erastin is selectively lethal to oncogenic RAS-mutant cell lines, and triggers a unique iron-dependent form of non-apoptotic cell death called ferroptosis. [1] [2]

Erastin binds directly to VDAC2 and causes mitochondrial damage via ROS production in an NADH-dependent manner, which induces cell death in some tumor cells harbouring activating mutations in the RAS-RAF-MEK pathway. [3]

In addition, erastin, via inducing ROS-mediated CID (Caspase-independent cell death), strongly enhances the effect in WT EGFR cells. [4]
In vivo

Erastin is a ferroptosis activator by inhibiting voltage-dependent anion channels (VDAC2/VDAC3). It also inhibits cystine-glutamate antiporter (xCT).[5]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    BJ-TERT/LT/ST/RASV12 cells

  • Concentrations

    5 or 10 μg/mL

  • Incubation Time

    6-11 hours

  • Method

    BJ-TERT/LT/ST/RASV12 cells are seeded in 100 mm dishes and allowed to grow overnight. Cells are treated with erastin (5 or 10 μg/ml) for 6, 8, or 11 hr. A camptothecin-treated (0.4 μg/ml) control is maintained, treated at the time of seeding for 20 hours. After the treatment, cells are harvested with trypsin/EDTA and washed once with fresh medium containing serum and then twice with phosphate-buffered saline. Cells are resuspended in 1× binding buffer. 100 μL is incubated with 5 μL of Annexin V-FITC and propidium iodiode for 15 min in the dark at room temperature. Then 400 μl of the 1× binding buffer s added and the cells analyzed by flow cytometry. Data are acquired and analyzed using Cellquest software. Only viable cells that do not stain with propidium iodiode are analzyed for Annexin V-FITC staining using the FL1 channel.
Animal Study:

[5]

 
  • Animal Models

    C57BL/6 mice

  • Dosages

    10 mg/kg

  • Administration

    i.n.

Customer Product Validation

, , Cancer Res, 2017, 77(8):2064-2077

Data from [Data independently produced by , , Gastroenterology, 2018, 154(5):1480-1493]

Data from [Data independently produced by , , Gastroenterology, 2017, 153(5):1429-1443]

Data from [Data independently produced by , , Hepatology, 2016, 64(2):488-500.]

Selleck's Erastin has been cited by 472 publications

Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models [ Nat Commun, 2025, 16(1):1982] PubMed: 40011434
S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism [ Nat Commun, 2025, 16(1):509] PubMed: 39779666
A transcriptional atlas of gut-innervating neurons reveals activation of interferon signaling and ferroptosis during intestinal inflammation [ Neuron, 2025, S0896-6273(25)00136-9] PubMed: 40101721
Inhibition of Endoplasmic Reticulum Stress Cooperates with SLC7A11 to Promote Disulfidptosis and Suppress Tumor Growth upon Glucose Limitation [ Adv Sci (Weinh), 2025, 12(7):e2408789] PubMed: 39739602
SLC25A1 and ACLY maintain cytosolic acetyl-CoA and regulate ferroptosis susceptibility via FSP1 acetylation [ EMBO J, 2025, 10.1038/s44318-025-00369-5] PubMed: 39881208
VIPAS39 confers ferroptosis resistance in epithelial ovarian cancer through exporting ACSL4 [ EBioMedicine, 2025, 114:105646] PubMed: 40088627
LncRNA CRCMSL interferes in phospholipid unsaturation to suppress colorectal cancer progression via reducing membrane fluidity [ J Adv Res, 2025, S2090-1232(25)00075-X] PubMed: 39921055
Exosomal miR-375-3p mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN [ Int J Biol Sci, 2025, 21(3):1275-1293] PubMed: 39897035
Inhibition of KLF5 promotes ferroptosis via the ZEB1/HMOX1 axis to enhance sensitivity to oxaliplatin in cancer cells [ Cell Death Dis, 2025, 16(1):28] PubMed: 39827156
Targeting PTGDS Promotes ferroptosis in peripheral T cell lymphoma through regulating HMOX1-mediated iron metabolism [ Br J Cancer, 2025, 132(4):384-400] PubMed: 39706989

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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