MCC950 Sodium

Catalog No.S7809 Batch:S780908

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Technical Data

Formula

C20H23N2O5S.Na
Molecular Weight 426.46 CAS No. 256373-96-3
Solubility (25°C)* In vitro Water 43 mg/mL (100.83 mM)
Ethanol 43 mg/mL (100.83 mM)
DMSO Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
saline
43.0mg/ml Taking the 1 mL working solution as an example, add 43 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description MCC950 Sodium is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.
Targets
NLRP3 [1]
(in BMDM (bone marrow derived macrophages))		 NLRP3 [1]
(in HMDM)
7.5nM 8.1 nM
In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]
In vivo

MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    H9c2 cardiomyocyte cells

  • Concentrations

    7 nM

  • Incubation Time

    24 h

  • Method

    Cardiomyocytes were treated with NLRP3 inhibitor MCC950 for 24 h.
Animal Study:

[1]
  • Animal Models

    C57BL/6 mice

  • Dosages

    50 mg/kg

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , ARTHRITIS & RHEUMATOLOGY, 2017, 69(8):1636-1646]

Data from [Data independently produced by , , Biochem Pharmacol, 2018, 154:278-292]

Data from [Data independently produced by , , Biochem Biophys Res Commun, 2018, 503(4):3031-3037]

Data from [Data independently produced by , , BMC Complement Altern Med, 2018, 18(1):192]

Selleck's MCC950 Sodium has been cited by 145 publications

Tonic TCR and IL-1β signaling mediate phenotypic alterations of naive CD4+ T cells [ Cell Rep, 2024, 43(3):113954] PubMed: 38492221
A stress sensor, IRE1α, is required for bacterial-exotoxin-induced interleukin-1β production in tissue-resident macrophages [ Cell Rep, 2024, 43(4):113981] PubMed: 38520688
sTREM2 Differentially Affects Cytokine Expression in Myeloid-Derived Cell Models via MAPK-JNK Signaling Pathway [ Biology (Basel), 2024, 13(2)87] PubMed: 38392305
Force-induced Caspase-1-dependent pyroptosis regulates orthodontic tooth movement [ Int J Oral Sci, 2024, 16(1):3] PubMed: 38221531
Clostridioides difficile aggravates dextran sulfate solution (DSS)-induced colitis by shaping the gut microbiota and promoting neutrophil recruitment [ Gut Microbes, 2023, 15(1):2192478] PubMed: 36951545
Long-term exposure to house dust mites accelerates lung cancer development in mice [ J Exp Clin Cancer Res, 2023, 42(1):26] PubMed: 36670473
The involvement of NLRP3 inflammasome in CUMS-induced AD-like pathological changes and related cognitive decline in mice [ J Neuroinflammation, 2023, 20(1):112] PubMed: 37165444
The microbiota regulates hematopoietic stem and progenitor cell development by mediating inflammatory signals in the niche [ Cell Rep, 2023, 42(2):112116] PubMed: 36795566
Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays [ Cell Chem Biol, 2023, S2451-9456(23)00335-5] PubMed: 37858335
PARP1 promotes NLRP3 activation via blocking TFEB-mediated autophagy in rotenone-induced neurodegeneration [ Ecotoxicol Environ Saf, 2023, 252:114630] PubMed: 36764072

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.