Mitomycin C

Catalog No.S8146 Batch:S814604

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Technical Data

Formula

C15H18N4O5
Molecular Weight 334.37 CAS No. 50-07-7
Solubility (25°C)* In vitro DMSO 67 mg/mL (200.37 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
Targets
DNA synthesis [1]
In vitro

Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. [1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. [2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. [3]
In vivo

As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. [4]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    HCT116 cells

  • Concentrations

    5 µM

  • Incubation Time

    24 h

  • Method

    Cells were pre-treated with 5 µM MMC for 24 h and then co-treated with TRAIL (25 ng/mL).
Animal Study:

[4]
  • Animal Models

    Rat bladder tumor model

  • Dosages

    400 μM

  • Administration

    Intravesical treatment

Customer Product Validation

Data from [Data independently produced by , , Cancer Sci, 2018, 109(11):3450-3460]

Selleck's Mitomycin C has been cited by 114 publications

Identifying squalene epoxidase as a metabolic vulnerability in high-risk osteosarcoma using an artificial intelligence-derived prognostic index [ Clin Transl Med, 2024, 14(2):e1586] PubMed: 38372422
Culture and expansion of murine proximal airway basal stem cells [ Stem Cell Res Ther, 2024, 15(1):26] PubMed: 38287366
Exosomal miR-4645-5p from hypoxic bone marrow mesenchymal stem cells facilitates diabetic wound healing by restoring keratinocyte autophagy [ Burns Trauma, 2024, 12:tkad058] PubMed: 38250706
Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application [ Cell Rep Med, 2023, 10.1016/j.xcrm.2023.101277] PubMed: 37944531
PGF2α facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR+ CXC chemokine expression [ EMBO Mol Med, 2023, 15(1):e16373] PubMed: 36511116
PGF2α facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR+ CXC chemokine expression [ EMBO Mol Med, 2023, 15(1):e16373] PubMed: 36511116
Targeting TCF19 sensitizes MSI endometrial cancer to anti-PD-1 therapy by alleviating CD8+ T cell exhaustion via TRIM14-IFN-β axis [ Cell Rep, 2023, 42(8):112944] PubMed: 37566545
Demethylating therapy increases cytotoxicity of CD44v6 CAR-T cells against acute myeloid leukemia [ Front Immunol, 2023, 14:1145441] PubMed: 37180104
T-cell dysfunction in natural killer/T-cell lymphoma [ Oncoimmunology, 2023, 12(1):2212532] PubMed: 37250921
Screening of Natural Compounds for CYP11A1 Stimulation Against Cell Renal Cell Carcinoma [ Biol Proced Online, 2023, 25(1):31] PubMed: 38036976

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.