Nortriptyline hydrochloride

Catalog No.S3698 Batch:S369801

Technical Data

Formula	C₁₉H₂₁N.HCl
Molecular Weight	299.84	CAS No.	894-71-3
Solubility (25°C)*	In vitro	DMSO	59 mg/mL (196.77 mM)
		Water	59 mg/mL (196.77 mM)
		Ethanol	59 mg/mL (196.77 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Nortriptyline hydrochloride (Desitriptyline, ELF-101, EN-7048, Desmethylamitriptyline) is the hydrochloride salt form of nortriptyline, a tricyclic antidepressant agent used for short-term treatment of various forms of depression.
In vitro	Nortriptyline inhibits the vascular Kv channels in a concentration-dependent and state-independent manner independently of serotonin reuptake inhibition^[1]. It is a tricyclic antidepressant. At the cellular level, nortriptyline is inhibitory to many proteins and responses such as astroglial inwardly rectifying Kir4.1 channels, human neutrophil phagocytosis and oxidative burst, human cytochrome P-450 enzymes, opioid receptors, Ca2+-activated K+ channels, and priming of human neutrophils. Nortriptyline induces a significant [Ca2+]i rise and decreases viability in PC3 cells, MG63 human osteosarcoma, and renal tubular cells^[2]. Nortriptyline also exhibits anticancer activity in several different types of cells. For example,in human cutaneous melanoma cells,NTP has a half maximal inhibitory concentration(IC50) of 9 μM compared with 27 μM and 33 μM for clomipramine and amitriptyline, respectively. Nortriptyline induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells^[3].
In vivo	Nortriptyline inhibits tumor growth in mice inoculated with MBT-2 cells. Low concentrations of NTP (0.1-5 μM) confer neuroprotective effects, downregulate cytosolic phospholipase A2, and prevent mitochondrial depolarization with minimal toxicity in both astrocytes and mice. In contrast, higher concentrations of NTP (10-50 μM) have anti-tumor effects on human osteosarcoma and cutaneous melanoma cells^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines PC3 cells Concentrations 50-500 µM Incubation Time 24 h Method Measurement of cell viability is based on the ability of cells to cleave tetrazolium salts by dehydrogenases. Augmentation in the amount of developed color directly correlates with the number of live cells. Cells are seeded in 96-well plates at 10,000 cells/well in culture medium for 24 h in the presence of several concentrations of nortriptyline. The cell viability-detecting reagent WST-1 is added to samples after nortriptyline treatment, and cells are incubated for 30 min in a humidified atmosphere.
Animal Study:^{^[3]}	Animal Models male C3H/HeN mice Dosages 10 or 20 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
PC3 cells
Concentrations
50-500 µM
Incubation Time
24 h
Method
Measurement of cell viability is based on the ability of cells to cleave tetrazolium salts by dehydrogenases. Augmentation in the amount of developed color directly correlates with the number of live cells. Cells are seeded in 96-well plates at 10,000 cells/well in culture medium for 24 h in the presence of several concentrations of nortriptyline. The cell viability-detecting reagent WST-1 is added to samples after nortriptyline treatment, and cells are incubated for 30 min in a humidified atmosphere.

Animal Study:^{^[3]}

Animal Models
male C3H/HeN mice
Dosages
10 or 20 mg/kg
Administration
i.p.

References

Selleck's Nortriptyline hydrochloride has been cited by 1 publication

Differential Interactome Based Drug Repositioning Unraveled Abacavir, Exemestane, Nortriptyline Hydrochloride, and Tolcapone as Potential Therapeutics for Colorectal Cancers [ Front Bioinform, 2021, 1:710591]	PubMed: 36303724

Differential Interactome Based Drug Repositioning Unraveled Abacavir, Exemestane, Nortriptyline Hydrochloride, and Tolcapone as Potential Therapeutics for Colorectal Cancers [ Front Bioinform, 2021, 1:710591]

PubMed: 36303724

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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