Oligomycin A

Catalog No.S1478 Batch:S147806

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Technical Data

Formula

C45H74O11
Molecular Weight 791.06 CAS No. 579-13-5
Solubility (25°C)* In vitro DMSO 100 mg/mL (126.41 mM)
Water ˂1 mg/mL
Ethanol ˂1 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Oligomycin A (MCH 32), a dominant analog of the isomers, is an inhibitor of mitochondrial F1FO ATP synthase which inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. It inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. It also induces apoptosis in a variety of cell types.
Targets
ATP synthase [1]
(Cell-free assay)
In vitro

Oligomycin A is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.Oligomycin A inhibits ATP synthase by blocking its proton channel (Fo subunit), which is necessary for oxidative phosphorylation of ADP to ATP. The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. [1]

In a group of cancer cells, Oligomycin at 100 ng/ml completely inhibits oxidative phosphorylation activity in 1 h and induces various levels of glycolysis gains by 6 h. [2]

Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis. [3]

Oligomycin inhibits mitochondrial respiration in melanoma, sensitizes melanoma cells to therapy, blocks the emergence of the slow-cycling, long-term tumor-maintaining melanoma cells. [4]
In vivo

Oligomycin A (MCH 32) is an inhibitor of mitochondrial F0F1-ATPase.

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    H1229

  • Concentrations

    100 ng/ml

  • Incubation Time

    1 h

  • Method

    ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
Animal Study:

[5]
  • Animal Models

    Male Sprague-Dawley rats

  • Dosages

    0.5 mg/kg

  • Administration

    i.v.

Customer Product Validation

Data from [Data independently produced by , , Sci Rep, 2016, 6:24641.]

Data from [Data independently produced by , , Int J Oncol, 2018, 53(6):2590-2604]

Data from [Data independently produced by , , PLoS One, 2017, 12(4):e0176355]

Selleck's Oligomycin A has been cited by 90 publications

AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis [ Nat Commun, 2024, 15(1):686] PubMed: 38263319
Dendritic cells overcome Cre/Lox induced gene deficiency by siphoning cytosolic material from surrounding cells [ iScience, 2024, 27(3):109119] PubMed: 38384841
Ketogenesis promotes tolerance to Pseudomonas aeruginosa pulmonary infection [ Cell Metab, 2023, 35(10):1767-1781.e6] PubMed: 37793346
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice [ Nat Commun, 2023, 14(1):2523] PubMed: 37130842
Alanine supplementation exploits glutamine dependency induced by SMARCA4/2-loss [ Nat Commun, 2023, 14(1):2894] PubMed: 37210563
Adamantaniline Derivatives Target ATP5B to Inhibit Translation of Hypoxia Inducible Factor-1α [ Adv Sci (Weinh), 2023, 10(25):e2301071] PubMed: 37401167
Deciphering a global source of non-genetic heterogeneity in cancer cells [ Nucleic Acids Res, 2023, gkad666] PubMed: 37587722
Fucoidan from Fucus vesiculosus prevents the loss of dopaminergic neurons by alleviating mitochondrial dysfunction through targeting ATP5F1a [ Carbohydr Polym, 2023, 303:120470] PubMed: 36657849
CRISPR/Cas-Assisted Nanoneedle Sensor for Adenosine Triphosphate Detection in Living Cells [ ACS Appl Mater Interfaces, 2023, 10.1021/acsami.3c07918] PubMed: 37769296
Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway [ Int J Biol Sci, 2023, 19(7):2006-2019] PubMed: 37151872

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.