Rituximab (anti-CD20)

Catalog No.A2009 Batch: A200904

Technical Data

CAS No.	174722-31-7
Formulation	PBS buffer, pH 7.2
Isotype	Human IgG1
Source	CHO cells
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity	99%
Protein concentration	7.28mg/ml
Endotoxin Level	<1 EU/mg

Biological Activity

Description

Rituximab (anti-CD20) is a chimeric anti-CD20 mAb that binds the CD20 antigen on B cells with a binding affinity of 5 nM, MW: 143.86 KD.

Targets

CD20 ^[1]

In vitro

Anti-CD20 IgG1 antibody (rituximab), induces strong antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) effects on CD20+ Raji cells with the EC50 at 0.0365 nM and 1.5 nM, respectively.^[1]

In vivo

A number of in vivo tumor models suggest the anti-tumor activity of rituximab is dependent, at least in part, on complement^[2]. Rituximab can deplete B cells for several months and, as such, could represent an effective therapy for B cell-mediated autoimmune diseases. Rituximab is now widely used in onco-haematology and is currently in development in several autoimmune diseases^[3].

Protocol (Only for Reference)

Cell Assay:	For ADCC experiments, PBMCs were isolated from healthy human donor blood to serve as effector cells and were plated with target Raji cells at E:T ratio of 50:1 in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Target Raji cells were labeled with calcein-AM for detection of cell lysis prior to experimental set up. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed. For CDC experiments, human complement was plated with calcein-AM labeled target Raji cells in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed.
Animal Study:	Objective: To study the mechanisms of follicular lymphoma’s resistance to rituximab Animal Models: Female CB17 severe combined immunodeficient(SCID) mice were injected (s.c.) with rituximab-resistant lymphoma cells (derived from a human FL) Formulation: -- Dosages: 10mg/kg, weekly Administration: i.p. Reference: https://www.ncbi.nlm.nih.gov/pubmed/19188155 Objective: To investigate the therapeutic effect of rituximab in combination with L19-IL2 on B-cell malignancies Animal Models: CB17/lcr severe combined mmunodeficiency (SCID) mice were injected (s.c.) Ramos or DoHH-2 lymphoma cells Formulation: saline Dosages: 200 μg Administration: i.v. Reference: https://www.ncbi.nlm.nih.gov/pubmed/19005180 Rituximab can apply to immunodeficient mice (eg: SCID mice), lymphoma cell lines and other related assays (Only for Reference)

Cell Assay:

For ADCC experiments, PBMCs were isolated from healthy human donor blood to serve as effector cells and were plated with target Raji cells at E:T ratio of 50:1 in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Target Raji cells were labeled with calcein-AM for detection of cell lysis prior to experimental set up. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed. For CDC experiments, human complement was plated with calcein-AM labeled target Raji cells in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed.

Animal Study:

Objective: To study the mechanisms of follicular lymphoma’s resistance to rituximab
Animal Models: Female CB17 severe combined immunodeficient(SCID) mice were injected (s.c.) with rituximab-resistant lymphoma cells (derived from a human FL)
Formulation: --
Dosages: 10mg/kg, weekly
Administration: i.p.
Reference: https://www.ncbi.nlm.nih.gov/pubmed/19188155

Objective: To investigate the therapeutic effect of rituximab in combination with L19-IL2 on B-cell malignancies
Animal Models: CB17/lcr severe combined mmunodeficiency (SCID) mice were injected (s.c.) Ramos or DoHH-2 lymphoma cells
Formulation: saline
Dosages: 200 μg
Administration: i.v.
Reference: https://www.ncbi.nlm.nih.gov/pubmed/19005180

Rituximab can apply to immunodeficient mice (eg: SCID mice), lymphoma cell lines and other related assays (Only for Reference)

References	[1] Muralidharan S, et al. J Pharmacol Exp Ther. 2022 Apr;381(1):12-21. [2] George J. Weiner, et al. Semin Hematol. 2010, 47(2): 115-123. [3] Cartron G, et al. Crit Rev Oncol Hematol. 2007, 62(1):43-52.

References

Selleck's Rituximab (anti-CD20) has been cited by 11 publications

Noninvasive PET imaging of tumor PD-L1 expression with 64Cu-labeled Durvalumab [ Am J Nucl Med Mol Imaging, 2024, 14(1):31-40]	PubMed: 38500749
Daratumumab induces cell-mediated cytotoxicity of primary effusion lymphoma and is active against refractory disease [ Oncoimmunology, 2023, 12(1):2163784]	PubMed: 36632565
Development and implementation of natural killer cell simultaneous ADCC and direct killing assay [ Heliyon, 2023, 9(12):e22991]	PubMed: 38125417
Rheumatoid factor IgM autoantibodies control IgG homeostasis [ Front Immunol, 2022, 13:1016263]	PubMed: 36341420
Beta-Lapachone Attenuates BMSC-Mediated Neuroblastoma Malignant Transformation by Inhibiting Gal-3/Gal-3BP/IL6 Axis [ J Pharmacol Exp Ther, 2022, 381(1):12-21]	PubMed: 35078863
A versatile platform for the tumor-targeted delivery of immune checkpoint-blocking immunoglobin G [ J Control Release, 2021, 340:243-258]	PubMed: 34752799
Study of the antilymphoma activity of pracinostat reveals different sensitivities of DLBCL cells to HDAC inhibitors [ Blood Adv, 2021, 5(10):2467-2480]	PubMed: 33999145
Salt-dependent passive adsorption of IgG1κ-type monoclonal antibodies on hydrophobic microparticles [ Biophys Chem, 2021, 275:106609]	PubMed: 33975078
Tumor-Targeting Liposomes with Transient Holes Allowing Intact Rituximab Internally [ Biomacromolecules, 2020, 22(2):723-731]	PubMed: 33280388
Maternal embryonic leucine zipper kinase is a novel target for diffuse large B cell lymphoma and mantle cell lymphoma. [ Blood Cancer J, 2019, 9(12):87]	PubMed: 31740676

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.