Secukinumab (anti-IL-17A)

Catalog No.A2025        Batch: A202501

Print

Technical Data

CAS No. 1229022-83-6
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source Human
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99.82%
Protein concentration 5.00mg/ml
Endotoxin Level <1EU/mg

Biological Activity

Description Secukinumab (anti-IL-17A) (Cosentyx, AIN457) is a human IgG1κ monoclonal antibody that binds to the protein interleukin (IL)-17A, and is applied for the treatment of psoriasis, ankylosing spondylitis, and psoriatic arthritis. MW=147.9 kDa.
In vitro

Secukinumab can reduce cell growth in the miR130b-knockdown OCI-ly10 co-culture system.[1]
In vivo

Secukinumab administration significantly attenuates AAV8-IRF8 mediated recruitment of neutrophils, also downregulates the autophagy level and inhibits the release of either autophagy-dependent or independent chemokines in vivo.[2]

Protocol (Only for Reference)

Cell Assay:

  • MTT assay was adopted to measure cell growth in the control inhibitor or miR130b inhibitor transfected OCI-ly10 co-culture system upon treatment with or without IL17 inhibitor.
Animal Study:

  • Animal Models: C57BL/6 mice, IRF8 FLOX mice (C57BL/6J background)
    Dosages: 200 μg
    Administration: i.p.
    Reference: https://pubmed.ncbi.nlm.nih.gov/34400126/
     

Selleck's Secukinumab (anti-IL-17A) has been cited by 2 publications

Overexpression of microRNA-145 enhanced docetaxel sensitivity in breast cancer cells via inactivation of protein kinase B gamma-mediated phosphoinositide 3-kinase -protein kinase B pathway [ Signal Transduct Target Ther, 2022, 7(1):80] PubMed: 35301282
Hepatic interferon regulatory factor 8 expression mediates liver ischemia/reperfusion injury in mice [ Biochem Pharmacol, 2021, 192:114728] PubMed: 34400126

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.