Sodium Demethylcantharidate

Catalog No.S3861 Batch:S386101

Technical Data

Formula	C₈H₉O₅.Na
Molecular Weight	208.14	CAS No.	13114-29-9
Solubility (25°C)*	In vitro	Water	41 mg/mL (196.98 mM)
		DMSO	Insoluble

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Demethylcantharidin (norcantharidin) is a synthetic analogue of cantharidin and has potent antitumor activity.
In vitro	Demethylcantharidin (DMC) is also found to be highly effective in inhibiting the growth of mouse hepatocytes, with an IC50 of about 10 μM^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines SK-Hep1 and HepG2 cells Concentrations 2-200 μM Incubation Time 72 h Method Cells are seeded in 96-well microtiter plates (1×10⁴/well; 100 μl) and allowed to equilibrate in quadruplicate wells and exposed to drugs (cisplatin, 1-5 and DMC: 2-200 μM; carboplatin: 50-3000 μM) for 72 h. After which, MTT (5 mg/ml, 20 ml) in phosphate-buffered saline is added and the cells are incubated for 4 h at 37℃. MTT/medium is then removed and the formazan product is dissolved in DMSO (150 ml) and absorbance is measured at 570 nm using a microtiter plate reader

Cell Assay:^{^[1]}

Cell lines
SK-Hep1 and HepG2 cells
Concentrations
2-200 μM
Incubation Time
72 h
Method
Cells are seeded in 96-well microtiter plates (1×10⁴/well; 100 μl) and allowed to equilibrate in quadruplicate wells and exposed to drugs (cisplatin, 1-5 and DMC: 2-200 μM; carboplatin: 50-3000 μM) for 72 h. After which, MTT (5 mg/ml, 20 ml) in phosphate-buffered saline is added and the cells are incubated for 4 h at 37℃. MTT/medium is then removed and the formazan product is dissolved in DMSO (150 ml) and absorbance is measured at 570 nm using a microtiter plate reader

References

[1] To KK, et al. Anticancer Drugs. 2005, 16(8):825-35.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.