Tetracycline

Catalog No.S4490 Batch:S449003

Technical Data

Formula

C₂₂H₂₄N₂O₈

Molecular Weight

444.43

CAS No.

60-54-8

Solubility (25°C)*

In vitro

DMSO

89 mg/mL (200.25 mM)

Ethanol

22 mg/mL (49.5 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5% DMSO 1 95% Corn oil	0.55mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 11 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.45mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 89 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Tetracycline (TC) is a broad-spectrum antibiotic that exhibits activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites.
In vitro	Tetracycline-induced CK2 expression significantly increases the non–small cell lung cancer (NSCLC) invasion in cells expressing BRMS1 wild-type, but not the S30A mutant.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines H157 BRMS1 knockdown (KD) cells, H157 stable cells Concentrations 1 µg/mL Incubation Time 48 h Method H157 BRMS1 knockdown (KD) cells were cotransfected with pcDNA3-luciferase and Tet-on CK2α'. Stable clones were selected by Geneticin (400 µg/mL), Blasticidin (5 µg/mL), and Zeocin (150 µg/mL). Myc/His(6)- CK2α' expression was confirmed by immunoblot after treatment with TCN (1 µg/mL) for 48 h. Then, the H157 BRMS1^KD/luciferase/Tet-on CK2α' cells were infected with pBabe-shRNA–resistant FLAG-BRMS1 wild-type, S30A mutant, or empty vector and selected with puromycin (1 µg/mL). Flag-BRMS1 expression was confirmed by Western blot. H157 stable cells were pretreated with or without TCN (1 µg/mL) for 48 h. the inserts were coated with type IV collagen at a concentration of 0.25 mg/ml. The bottom portion of the wells included 1% FBS-media. Cells were allowed to migrate for 6 hours or invade for 22 hours. At the specified time point, inserts were fixed and stained with crystal violet.

Cell Assay:^{^[2]}

Cell lines
H157 BRMS1 knockdown (KD) cells, H157 stable cells
Concentrations
1 µg/mL
Incubation Time
48 h
Method
H157 BRMS1 knockdown (KD) cells were cotransfected with pcDNA3-luciferase and Tet-on CK2α'. Stable clones were selected by Geneticin (400 µg/mL), Blasticidin (5 µg/mL), and Zeocin (150 µg/mL). Myc/His(6)- CK2α' expression was confirmed by immunoblot after treatment with TCN (1 µg/mL) for 48 h. Then, the H157 BRMS1^KD/luciferase/Tet-on CK2α' cells were infected with pBabe-shRNA–resistant FLAG-BRMS1 wild-type, S30A mutant, or empty vector and selected with puromycin (1 µg/mL). Flag-BRMS1 expression was confirmed by Western blot. H157 stable cells were pretreated with or without TCN (1 µg/mL) for 48 h. the inserts were coated with type IV collagen at a concentration of 0.25 mg/ml. The bottom portion of the wells included 1% FBS-media. Cells were allowed to migrate for 6 hours or invade for 22 hours. At the specified time point, inserts were fixed and stained with crystal violet.

References

Selleck's Tetracycline has been cited by 1 publication

Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [ PeerJ, 2022, 10:e13719]	PubMed: 35846878

Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [ PeerJ, 2022, 10:e13719]

PubMed: 35846878

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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