Tocilizumab (anti-IL-6R)

Catalog No.A2012        Batch: A201202

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Technical Data

CAS No. ‎375823-41-9
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 98.85%
Protein concentration 4.97mg/ml
Endotoxin Level ≤1 EU/mg

Biological Activity

Description Tocilizumab (anti-IL-6R) is a humanized monoclonal antibody that binds to the interleukin-6 receptor, MW: 148 KD.
Targets
IL-6R [1]
In vitro

After adding tocilizumab at different concentrations into HMO6 and CHME5 cells stimulated by F3-CM or with IL6, the expression levels of anti-inflammatory M2 markers (CD206 and Arg1) are decreased, as well as the levels of both phosphorylated JAK2 and STAT3.[1]
In vivo

Tocilizumab can block JAK2/STAT3 activation and impede brain metastasis (BM) of A549-F3 cells in vivo.[1]

Protocol (Only for Reference)

Cell Assay:

  • HMO6 and CHME5 cells with control media or F3-CM alone or F3-CM were treated with Tocilizumab (2.5 μg/ml for 24 h) or with IL6 (200 ng/ml for 24 h) alone or IL6-supplemented with Tocilizumab. Western blot analysis of p-JAK2, JAK2, p-STAT3, and STAT3 in HMO6 and CHME5 cells were conducted.
Animal Study:

  • Animal Models: Nude mice of brain metastasis models
    Dosages: 5 mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864012/
     

Selleck's Tocilizumab (anti-IL-6R) has been cited by 42 publications

Autoimmunity in Down's syndrome via cytokines, CD4 T cells and CD11c+ B cells [ Nature, 2023, 615(7951):305-314] PubMed: 36813963
Peripheral helper-T-cell-derived CXCL13 is a crucial pathogenic factor in idiopathic multicentric Castleman disease [ Nat Commun, 2023, 14(1):6959] PubMed: 37907518
Anti-inflammatory effect of interleukin-6 highly enriched in secretome of two clinically relevant sources of mesenchymal stromal cells [ Front Cell Dev Biol, 2023, 11:1244120] PubMed: 37745306
Cancer-associated fibroblasts induce growth and radioresistance of breast cancer cells through paracrine IL-6 [ Cell Death Discov, 2023, 9(1):6] PubMed: 36635302
The Combination of a Human Biomimetic Liver Microphysiology System with BIOLOGXsym, a Quantitative Systems Toxicology (QST) Modeling Platform for Macromolecules, Provides Mechanistic Understanding of Tocilizumab- and GGF2-Induced Liver Injury [ Int J Mol Sci, 2023, 24(11)9692] PubMed: 37298645
ENO1 Promotes OSCC Migration and Invasion by Orchestrating IL-6 Secretion from Macrophages via a Positive Feedback Loop [ Int J Mol Sci, 2023, 24(1)737] PubMed: 36614179
Anti-inflammatory effect of interleukin-6 highly enriched in secretome of two clinically relevant sources of mesenchymal stromal cells [ Front Cell Dev Biol, 2023, 11:1244120] PubMed: 37745306
IL-6 Plays a Critical Role in Stromal Fibroblast RANKL Induction and Consequent Osteoclastogenesis in Ameloblastoma Progression [ Lab Invest, 2023, 103(1):100023] PubMed: 36748192
Characterization of HZ0412a, a novel potent humanized anti-IL-6 receptor antibody that blocks IL-6R binding to gp130 [ Antib Ther, 2023, 6(2):119-126] PubMed: 37324549
Overexpression of microRNA-145 enhanced docetaxel sensitivity in breast cancer cells via inactivation of protein kinase B gamma-mediated phosphoinositide 3-kinase -protein kinase B pathway [ Signal Transduct Target Ther, 2022, 7(1):52] PubMed: 35194016

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

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