Anti-HA magnetic beads

For research use only.

Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody.

Selleck's has been cited by 66 publications

Advantages

Time saving: save 15-30 min comparing with agarose beads.

Simple operation: Magnetic separation and centrifugation-free.

High protein loading capacity.

High specificity.

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Description

Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody. With high loading of HA-tagged protein (more than 0.6 mg protein/mL) and high specificity, it is recommended to use for co-immunoprecipitation and protein purification.

Properties

Antibody isotype	Mouse IgG2b
Antibody purification	Protein A purified
Application	Co-Immunoprecipitation and protein Purification
Recommended volume	IP: 20 μl beads for 200 μl crude protein solution
Binding capacity	Minimum 0.6 mg protein eluted per ml of magnetic beads
Shipped in	Blue ice

Storage (From the date of receipt)

Store at 2-8°C for 2 years. DO NOT freeze or centrifuge Magnetic Beads.

Protocol

Trouble Shooting

Problem	Possible Reason	Suggested Improvement
High background	Nonspecifically binding of proteins to the antibody, megnetic beads or EP tubes	Pre-clear lysate to remove nonspecific binding proteins. After suspending beads for the final wash, transfer the entire sample to a clean EP tube and then centrifugation.
High background	Washing times are not sufficient.	Increase the number of washes. Increase duration time of washes.
No signal is observed.	HA tagged protein is not expressed in the sample.	Make sure the protein of interest contains the HA sequence. Prepare the fresh lysate. Use appropriate protease inhibitors.
	Incubation times are inadequate.	Increase the incubation times.
	Interfering substance is present in sample.	The lysate may contain high concentrations of dithiothreitol (DTT), 2-mercaptoethanol, or other reducing agents. Excessive detergent concentration may interfere with the antibody-antigen interaction.

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