Home Bioreagents Phosphatase Inhibitor Cocktail (2 Tubes, 100X)

Phosphatase Inhibitor Cocktail (2 Tubes, 100X)

For research use only.

For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.

Phosphatase Inhibitor Cocktail

Selleck's has been cited by 476 publications

Price Comparison

Price Contrast Calculation
Brand Cat. No. Type Packaging Price Work Volume (1x) 1 rxn PRICE
(for 1mL lysis system) 		Small Package
(100mL 1x) 		Large Package
(1000mL 1x)
Selleck B15001 2 Tubes (A in H2O, B in DMSO) 1mL A + 1mL B (100x) 37 USD 100mL 0.37 USD 37 USD
Sigma P5726-1ML Aqueous solution 1mL(100x) 65 USD 100mL 0.65 USD 65 USD
PIERCE 78420 Aqueous solution 1mL(100x) 107 USD 100mL 1.1 USD 107 USD
Calbiochem 524625-1ML Aqueous solution 1mL(100x) 120 USD 100mL 1.2 USD 120 USD
Roche 04906845001 Tablets 10 Tablets
(10x per tablet)		 150 USD 100mL 1.5 USD 150 USD
Selleck B15002 2 Tubes
(A inH2O,
B in DMSO)		 1mL A + 1mL B
(100x)x 10		 270 USD 1000mL 0.27 USD 270 USD
Sigma P5726-5ML Aqueous solution 5mL(100x) 222 USD 500mL 0.44 USD 444 USD
PIERCE 78427 Aqueous solution 10mL(100x) 730 USD 1000mL 0.73 USD 730 USD
Calbiochem 524625-1SET Aqueous solution 1mL x 5(100x) 468 USD 500mL 0.94 USD 936 USD
Roche 04906837001 Tablets 20 Tablets
(10x per tablet)		 180 USD 200mL 0.90 USD 900 USD

Description

For Phosphorylation Protection, more inhibitors mean better results. So we provide Two Tubes with 8 inhibitors.

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. For decades, researchers have used easily-accessible phosphate mimetics (tube A) to prevent the loss of phosphorylation on biomolecules in vitro. In recent years however, a number of naturally derived agents (tube B) have been discovered as potent inhibitors of phosphatases, thus increasing the available protection spectrum.

Contents

Tube # Ingredient 100x Conc. Target
A (Aqueous) Sodium Fluoride 100 mM Acid phosphatases
Sodium Orthovanadate 100 mM Alkaline phosphatases, PTPs, ATPases
Sodium Tartrate 400 mM Acid phosphatases
Sodium Molybdate 115 mM Acid and phosphoprotein phosphatases
Imidazole 200 mM Alkaline phosphatases
B (DMSO) (-)-p-Bromotetramisole oxalate 2.5 mM Alkaline phosphatases
Cantharidin 500 μM Ser/Thr phosphatases
Microcystin LR, Microcystis aeruginosa 500 nM PP1 and PP2A

Works well in common detergents such as 1% SDS, Triton, and NP-40
DTT, EDTA and EGTA may reduce the protection effects of Sodium Orthovanadate

Storage (From the date of receipt)

The product should be stored at -20°C, and is stable for up to 2 years. When kept at 2-8°C, the product remains stable for 2 months.

Protocol

Application Fields Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Experiment Protocol Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Note:

For optimum protection of phosphorylated proteins and lipids, we recommend simultaneous use of BOTH Tubes A and B. Because the inhibitor cocktails target different families of phosphatases, synergistic stabilization effects are observed with dual-treatment.

The Phosphatase Inhibitor Cocktail does not contain protease inhibitors. Therefore,we recommend that a protease inhibitor cocktail is added beforehand. Protease inhibitor cocktails are frequently used in mammalian cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

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Trouble Shooting

Question 1:
Weak western bands for both phosphorylated and non-phosphorylated proteins.

Answer:
Add a protease inhibitor cocktail beforehand to protect all kinds of proteins. Selleck provides high quality protease inhibitor cocktails at competitive prices.

Question 2:
Light-green sediment precipitated after mixing two tubes before use.

Answer:
This sediment is mainly salt precipitation. Salts in the aqueous tube and natural compounds in the DMSO tube will both be influenced by mixing. The precipitate cannot be completely re-dissolved and mixing the two tubes should be avoided. Separate addition of the two tubes is required as described in the instructions.

Question 3:
Tube B takes a long time to melt.

Answer:
This is because DMSO thaws very slowly, especially in low temperatures. Thawing in 25℃ water bath is recommended. Dividing Tube B solution into 100ul aliquots can shorten the thawing time.