Home Metabolism GTPCH inhibitor 2,4-Diamino-6-hydroxypyrimidine For research use only.

2,4-Diamino-6-hydroxypyrimidine

Synonyms: DAHP

2,4-Diamino-6-hydroxypyrimidine (DAHP) is a specific inhibitor for GTP cyclohydrolase I, the rate-limiting enzyme in de novo pterin synthesis.

2,4-Diamino-6-hydroxypyrimidine Chemical Structure

2,4-Diamino-6-hydroxypyrimidine Chemical Structure

CAS: 56-06-4

Selleck's 2,4-Diamino-6-hydroxypyrimidine has been cited by 2 publications

Purity & Quality Control

Batch: S368801 DMSO] 25 mg/mL] false] Water] 6 mg/mL] false] Ethanol] Insoluble] false Purity: 99.91%
99.91

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Biological Activity

Description 2,4-Diamino-6-hydroxypyrimidine (DAHP) is a specific inhibitor for GTP cyclohydrolase I, the rate-limiting enzyme in de novo pterin synthesis.
Targets
GTP cyclohydrolase I [1]
In vitro
In vitro 2,4-diamino-6-hydroxy-pyrimidine (DAHP), an inhibitor of GTP cyclohydrolase I, blocks the synthesis of tetrahydrobiopterin (BH4), which is a known cofactor of inducible nitric oxide synthase (iNOS). DAHP is shown to suppress the production of nitric oxide by cytokine-activated fibroblasts, smooth muscle cells or endothelial cells which could be attributed to its function as a cofactor antagonist. DAHP down-regulates the expression of iNOS protein and mRNA in a BH4-independent manner[1].
In Vivo
In vivo DAHP has no effect on brain serotonin levels, presumably due to poor brain penetration. DAHP causes a rapid decrease (T1/2 of less than 12 hr) in the BH4 levels in all tissues examined, except in the brain. The BH4 level returns to within the normal range less than 24 hr after cessation of the administration of DAHP, a finding which suggests that the BH4 level is in a dynamic steady state maintained by rapid local biosynthesis[2].
Animal Research Animal Models C57BL/6 mice
Dosages 3 g/kg/day
Administration oral

Chemical lnformation & Solubility

Molecular Weight 126.12 Formula

C4H6N4O
CAS No. 56-06-4 SDF Download 2,4-Diamino-6-hydroxypyrimidine SDF
Smiles C1=C(N=C(NC1=O)N)N
Storage (From the date of receipt) 3 years -20°C(in the dark) powder

In vitro
Batch:

DMSO : 25 mg/mL ( (198.22 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 6 mg/mL

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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