Home Immunology & Inflammation NADPH-oxidase inhibitor 2-Acetylphenothiazine (ML171) For research use only.

2-Acetylphenothiazine (ML171)

Synonyms: 2-APT

2-Acetylphenothiazine (ML171, 2-APT) is a potent and selective inhibitor of NADPH-oxidase with IC50s of 0.25 μM, 5 μM, 3μM, 5 μM and 5.5 μM for NOX1, NOX2, NOX3, NOX4 and xanthine oxidase, respectively.

2-Acetylphenothiazine (ML171) Chemical Structure

2-Acetylphenothiazine (ML171) Chemical Structure

CAS: 6631-94-3

Selleck's 2-Acetylphenothiazine (ML171) has been cited by 5 publications

Purity & Quality Control

Batch: S530401 DMSO] 48 mg/mL] false] ] ] false] ] ] false Purity: 99.99%
99.99

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Biological Activity

Description 2-Acetylphenothiazine (ML171, 2-APT) is a potent and selective inhibitor of NADPH-oxidase with IC50s of 0.25 μM, 5 μM, 3μM, 5 μM and 5.5 μM for NOX1, NOX2, NOX3, NOX4 and xanthine oxidase, respectively.
Targets
NOX1 [1]
(HEK293)		 NOX3 [1]
(HEK293)		 NOX2 [1]
(HEK293)		 NOX4 [1]
(HEK293)		 xanthine oxidase [1]
(HEK293)
0.25 μM 3 μM 5 μM 5 μM 5.5 μM
In vitro
In vitro ML171 is a potent and selective inhibitor of NOX1, with an IC50 of 129–156 nM in cell-based assays. ML171 is not cytotoxic, and is selective among NOX family members NOX2, NOX3, and NOX4, as well as against xanthine oxidase, another cellular source of ROS. In addition, ML171 is highly effective in inhibiting the cellular production of invadopodia in a human colon cancer cell line. It strongly blocks ROS generation in HT29 cells (IC50HT29 = 0.129 μM)[2]. ML171 does not inhibit mitochondrial ROS production[3].
Cell Research Cell lines DLD1 cells
Concentrations 10 μM
Incubation Time 1 h
Method

DLD1 cells were plated on glass coverslips and after 24hrs cells were transfected with active SrcYF or empty vector (mock). 48hrs after transfection, cells were treated as indicated for 1hr with 10 μM of ML171, or with DMSO or 10 μM DPI. Afterwards, cells were fixed and stained and visualized by confocal miscroscopy.

Chemical lnformation & Solubility

Molecular Weight 241.31 Formula

C14H11NOS
CAS No. 6631-94-3 SDF --
Smiles CC(=O)C1=CC2=C(C=C1)SC3=CC=CC=C3N2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 48 mg/mL ( (198.91 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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