Home Transmembrane Transporters TRP Channel activator - Calcium Channel inhibitor 2-APB (2-Aminoethyl Diphenylborinate) For research use only.

2-APB (2-Aminoethyl Diphenylborinate)

2-APB (2-Aminoethyl Diphenylborinate) is an IP3 receptor inhibitor and regulate IP3-induced calcium release.2-APB also inhibits the SOC channel activity and activates TRP channel at higher concentrations.

2-APB (2-Aminoethyl Diphenylborinate) Chemical Structure

2-APB (2-Aminoethyl Diphenylborinate) Chemical Structure

CAS: 524-95-8

Selleck's 2-APB (2-Aminoethyl Diphenylborinate) has been cited by 7 publications

Purity & Quality Control

Batch: Purity: 99.57%
99.57

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Biological Activity

Description 2-APB (2-Aminoethyl Diphenylborinate) is an IP3 receptor inhibitor and regulate IP3-induced calcium release.2-APB also inhibits the SOC channel activity and activates TRP channel at higher concentrations.
Targets
IP3 receptor [1] SOC channel [2] TRP channel [2]
In vitro
In vitro

2-APB, an inositol 1, 4, 5-trisphosphate receptor (IP3R) inhibitor, obviously attenuates the intracellular Ca2+ release and cardiomyocyte apoptosis induced by oxygen and glucose deprivation (OGD) stimulation and EndoA2 knockdown.[3]
Cell Research Cell lines neonatal rat cardiomyocytes
Concentrations 2 h
Incubation Time --
Method

Neonatal Sprague-Dawley rats (1 to 3 days old) are exposed to hypothermia until they became unresponsive and are then euthanized by cervical dislocation. Primary culture of NRCMs is performed. The cells are seeded at a density of 5 x 106 cells/ml in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 0.1 mM 5-bromodeoxyuridine. After 24 h, NRCMs are subjected to various treatments as described below. To assess the effects of EndoA2 on cardiac injury, the cells are transfected with EndoA2 siRNA or Ad-EndoA2. After transfection for 36 h, the cells are stimulated with oxygen and glucose deprivation (OGD) for another 12 h. Incubation of sodium phenylbutyrate (4-PBA), Cyclosporin A (CsA), 1,2-bis(o-aminophenoxy)ethane-N,N,N ,N -tetraacetic acid (BAPTA) or 2-Aminoethoxydiphenylborate (2-APB) is 2 h before OGD stimulation.Cardiomyocytes are cultured in serum-free, glucose and sodium pyruvate-free DMEM at 37 ℃ in an anoxia chamber saturated with 94% N2/5% CO2/ 1% O2.
In Vivo
In vivo

2-APB increases the basilar artery (BA) wall thickness and reduced the BA lumen diameter, inducing significant vascular changes, also alleviates cell apoptosis at 24 hours after SAH.[4]
Animal Research Animal Models Rats of subarachnoid hemorrhage (SAH) model
Dosages 0.5, 2 mg/kg
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 225.09 Formula

C14H16BNO
CAS No. 524-95-8 SDF --
Smiles B(C1=CC=CC=C1)(C2=CC=CC=C2)OCCN
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 45 mg/mL ( (199.92 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 23 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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