Home Ubiquitin SUMO inhibitor 2-D08 For research use only.

2-D08

Synonyms: 2',3',4'-trihydroxy flavone

2-D08 (2',3',4'-trihydroxy flavone) is a cell permeable, mechanistically unique inhibitor of protein sumoylation.

2-D08 Chemical Structure

2-D08 Chemical Structure

CAS: 144707-18-6

Selleck's 2-D08 has been cited by 13 publications

Purity & Quality Control

Batch: S869601 DMSO] 54 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.05%
99.05

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Biological Activity

Description 2-D08 (2',3',4'-trihydroxy flavone) is a cell permeable, mechanistically unique inhibitor of protein sumoylation.
Targets
sumoylation [2]
In vitro
In vitro

2-D08, a inhibitor of protein SUMOylation, enhances the all-trans retinoic acid (ATRA)-induced expression of key genes involved in differentiation, proliferation, and apoptosis in non-APL AML cells[1]. 2-D08 inhibits sumoylation by preventing transfer of SUMO from the UBC9-SUMO thioester to the substrate[2]. 
Cell Research Cell lines U937, HL60, THP1 cell lines
Concentrations 50 µM
Incubation Time 5days, 9 days
Method

Cells are treated for 5 days 2-D08 (50 µM). CD14, CD11b or CD15 expressions are assayed by western bolt or flow cytometry. Morphological changes of cells are shown by May-Grumwald-Giemsa (MGG)-stained. 

Chemical lnformation & Solubility

Molecular Weight 270.24 Formula

C15H10O5
CAS No. 144707-18-6 SDF --
Smiles C1=CC=C2C(=C1)C(=O)C=C(O2)C3=C(C(=C(C=C3)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 54 mg/mL ( (199.82 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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