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Amphotericin B

Name: Amphotericin B
Brand: Selleck Chemicals
SKU: S1636
Rating: 5 (12 reviews)

Synonyms: NSC 527017

Catalog No.S1636 Purity: 99.61%

Amphotericin B (AMB, NSC 527017) is an amphipathic polyene antibiotic which permeabilizes ergosterol-containing membranes.

Amphotericin B Chemical Structure

CAS: 1397-89-3

Selleck's Amphotericin B has been cited by 12 publications

Purity & Quality Control

Batch: Purity: 99.61%

99.61

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Biological Activity

Description

Amphotericin B (AMB, NSC 527017) is an amphipathic polyene antibiotic which permeabilizes ergosterol-containing membranes.

Targets

ergosterol ^[1]

In vitro
In vitro	Amphotericin B administration is limited by infusion-related toxicity, including fever and chills, an effect postulated to result from proinflammatory cytokine production by innate immune cells. Amphotericin B induces signal transduction and inflammatory cytokine release from cells expressing TLR2 and CD14. ^[1] Amphotericin B interacts with cholesterol, the major sterol of mammal membranes, thus limiting the usefulness of Amphotericin B due to its relatively high toxicity. Amphotericin B is dispersed as a pre-micellar or as a highly aggregated state in the subphase. ^[2] Amphotericin B only kills unicellular Leishmania promastigotes (LPs) when aqueous pores permeable to small cations and anions are formed. Amphotericin B (0.1 mM) induces a polarization potential, indicating K+ leakage in KCl-loaded liposomes suspended in an iso-osmotic sucrose solution. Amphotericin B (0.05 mM) exhibits a nearly total collapse of the negative membrane potential, indicating Na+ entry into the cells. ^[3]

In Vivo
In vivo	Amphotericin B results in prolonging the incubation time and decreasing PrPSc accumulation in the hamster scrapie model. Amphotericin B markedly reduces PrPSc levels in mice with transmissible subacute spongiform encephalopathies (TSSE). ^[4] Amphotericin B exerts a direct effect on Plasmodium falciparum and influences eryptosis of infected erythrocytes, parasitemia and hostsurvival in murine malaria. Amphotericin B tends to delay the increase of parasitemia and significantly delays host death plasmodium berghei-infected mice. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05593666	Recruiting	Primary Visceral Leishmaniasis	Drugs for Neglected Diseases\|Novartis Pharmaceuticals	December 27 2022	Phase 2
NCT05108545	Unknown status	Neutropenia and Fever	CSPC ZhongQi Pharmaceutical Technology Co. Ltd.	December 15 2021	Phase 3
NCT04993222	Completed	Bioequivalence	CSPC Zhongnuo Pharmaceutical (Shijiazhuang) Co. Ltd.	June 4 2020	Phase 1
NCT03905447	Terminated	Aspergillosis\|Lung Transplant Infection	Pulmocide Ltd	September 17 2019	Phase 2
NCT03828773	Recruiting	Candidiasis\|Fungal Infection\|Acute Myeloid Leukemia\|Genetic Predisposition\|Aspergillosis	Bochud Pierre-Yves\|Swiss National Science Foundation\|Centre Hospitalier Universitaire Vaudois	February 11 2019	Not Applicable

References

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Chemical lnformation & Solubility

Molecular Weight	924.08	Formula	C₄₇H₇₃NO₁₇
CAS No.	1397-89-3	SDF	Download Amphotericin B SDF
Smiles	CC1C=CC=CC=CC=CC=CC=CC=CC(CC2C(C(CC(O2)(CC(CC(C(CCC(CC(CC(=O)OC(C(C1O)C)C)O)O)O)O)O)O)O)C(=O)O)OC3C(C(C(C(O3)C)O)N)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 4.5 mg/mL ( (4.86 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 4.5 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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