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Crystal Violet

Name: Crystal Violet
Brand: Selleck Chemicals
SKU: S1917
Rating: 5 (6 reviews)

Synonyms: Gentian Violet

Catalog No.S1917 Purity: 99.81%

Crystal violet(Gentian Violet) is a triarylmethane dye.

Crystal Violet Chemical Structure

CAS: 548-62-9

Selleck's Crystal Violet has been cited by 6 publications

Purity & Quality Control

Dye content ≥90%
Finest quality approved by Nature Medicine
COA
Datasheet
SDS

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Biological Activity

Description	Crystal violet(Gentian Violet) is a triarylmethane dye.

In vitro
In vitro	1.Preparation of MitoSOX Red working solution Dilute the 2 g Crystal Violet in 20 mL 95% ethanol andmixed with 80 ml 1% ammonium oxalate solution solution for 24 h to obtain working solution. 2.Sample processing 2.1 Paraffin section: First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH2O 2 min. 2.2 Frozen section: distilled water for 2 min. 2.3 Tissue culture cells: fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min. Note: Other fixatives can be selected according to specific experiments. 3.Crystal violet staining 3.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results). 3.2 Wash well in distilled water. 3.3 Dry at room temperature. 3.4 Observe and photograph by light microscope.

Chemical lnformation & Solubility

Molecular Weight	407.98	Formula	C₂₅H₃₀ClN₃
CAS No.	548-62-9	SDF	Download Crystal Violet SDF
Smiles	CN(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vivo
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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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