Home DNA Damage/DNA Repair Antineoplastic and Immunosuppressive Antibiotics inhibitor - DNA/RNA Synthesis inhibitor Dacarbazine For research use only.

Dacarbazine

Synonyms: DTIC-Dome, Imidazole Carboxamide,NSC-45388

Dacarbazine is a triazene derivative with antineoplastic activity. Dacarbazine alkylates and cross-links DNA during all phases of the cell cycle, resulting in disruption of DNA function, cell cycle arrest, and apoptosis; used in the treatment of various cancers.

Dacarbazine Chemical Structure

Dacarbazine Chemical Structure

CAS: 4342-03-4

Selleck's Dacarbazine has been cited by 14 publications

Purity & Quality Control

Batch: Purity: 99.99%
99.99

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Biological Activity

Description Dacarbazine is a triazene derivative with antineoplastic activity. Dacarbazine alkylates and cross-links DNA during all phases of the cell cycle, resulting in disruption of DNA function, cell cycle arrest, and apoptosis; used in the treatment of various cancers.
Targets
Nucleoside antimetabolite/analog [1]
In vitro
In vitro Treatment of Dacarbazine sensitizes melanomas to lysis of peptide-specific CTL and it is is mediated through Fas-independent pathway[2].
Cell Research Cell lines UACC903 (UACC) cells
Concentrations 20 μM
Incubation Time 48 h
Method

Apotosis assay: UACC903 (UACC) cells treated with or without DTIC (20 μM) for 48 h are incubated with agonist anti-Fas Ab, CH-11 (500 ng/ml), for 5 and 16 h, respectively. To distinguish whether death/apoptosis is mediated by FasR, blocking anti-Fas Ab ZB4 (2 mg/ml) is added in the corresponding groups. At the end of incubation, cells are harvested and stained with propidium iodide and FITC-annexin V (BD PharMingen) for detecting apoptosis according to the instruction provided by the company. Ten thousand cells from each group are collected, and dead and apoptotic cells are analyzed without gating using CellQuest software.
Experimental Result Images Methods Biomarkers Images PMID
Western blot ATM / p-ATM / p21(Waf1/Cip1) / Survivin 27632933
Growth inhibition assay Cell viability 21976975
In Vivo
In vivo Treatment of combination of Axitinib and DTIC demonstrates significant antitumor activity against melanoma flank xenografts, reduces tumor cell proliferation and decreases the area of tumor necrosis and increases apoptosis. It also reduces meta-tasis-related factors and prolongs lifespan in mice[3].
Animal Research Animal Models B16F1 melanoma xenograft model(C57BL/6 mice background)
Dosages 80 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03761095 Active not recruiting Leiomyosarcoma PTC Therapeutics March 13 2019 Phase 1
NCT01974752 Completed Metastatic|Uveal Melanoma AstraZeneca April 2014 Phase 3

Chemical lnformation & Solubility

Molecular Weight 182.18 Formula

C6H10N6O
CAS No. 4342-03-4 SDF Download Dacarbazine SDF
Smiles CN(C)N=NC1=C(NC=N1)C(=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 3 mg/mL ( (16.46 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Could you please help me test some common vehicles for S1221 in vivo experiments?

Answer:
S1221 can dissolve in 0.5% CMC Na at 30mg/ml as a suspension. After stayed for a period of time, the powder will drop down to the bottom of the tube, so please vortex well before use.

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