Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Progesterone For research use only.

Progesterone

Synonyms: NSC 64377, Pregn-4-ene-3,20-dione,NSC 9704

Progesterone is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. A potent agonist of the nuclear progesterone receptor (nPR) with Kd of 1 nM; An agonist of the membrane progesterone receptors(mPRs); An antagonist of the σ1 receptor.

Progesterone Chemical Structure

Progesterone Chemical Structure

CAS: 57-83-0

Selleck's Progesterone has been cited by 10 Publications

2 Customer Reviews

Purity & Quality Control

Batch: Purity: 100%
100

Other Estrogen/progestogen Receptor Products

Related compound libraries

Choose Selective Estrogen/progestogen Receptor Inhibitors

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human T47D cells Function assay 20 h Activation of progesterone receptor in human T47D cells after 20 hrs by PRE-tagged luciferase reporter gene assay, EC50=0.1 nM 19863083
CV-1 cells Function assay Effective concentration for half-maximal activation of human progesterone receptor expressed in CV-1 cells, EC50=1.5 nM 9667968
HEK293 cells Function assay Agonist activity at human PRGR expressed in HEK293 cells by luciferase reporter gene assay, EC50=2 nM 25305688
SF-12 cells Function assay Displacement of DHT from human androgen receptor expressed in baculovirus SF-12 cells, Ki=9.5 nM 12954062
CHO cells Function assay Agonist activity at human TGR5 expressed in CHO cells by luciferase assay, EC50=2.77 μM 18307294
human K562/R7 cells Function assay 1 μM 72 h Potentiation of doxorubicin-induced cytotoxicity against doxorubicin-resistant human K562/R7 cells assessed as doxorubicin IC50 at 1 uM after 72 hrs by MTT assay, IC50=10.6 μM 25634041
A2780 cells Function assay Inhibition of P-gp in human adriamycin-resistant A2780 cells by Hoechst 33342 assay, IC50=47.863 μM 18678495
HeLa cells Cytotoxicity assay Cytotoxicity against human HeLa cells assessed as inhibition of DNA replication by imaging analysis 18066055
Click to View More Cell Line Experimental Data

Biological Activity

Description Progesterone is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. A potent agonist of the nuclear progesterone receptor (nPR) with Kd of 1 nM; An agonist of the membrane progesterone receptors(mPRs); An antagonist of the σ1 receptor.
Targets
progestogen Receptor [1]
In vitro
In vitro

Progesterone has biphasic effects on proliferation of breast cancer cells; it stimulates growth in the first cell cycle, then arrests cells at G1/S of the second cycle accompanied by up-regulation of the cyclin-dependent kinase inhibitor, p21. Progesterone-mediated transcription is further prevented by overexpression of E1A, suggesting that CBP/p300 is required. [1] Progesterone drives a series of events where luminal cells probably provide Wnt4 and RANKL signals to basal cells which in turn respond by upregulating their cognate receptors, transcriptional targets and cell cycle markers. [2] Progesterone treatment increases the sensitivity of cortical synaptoneurosomes to GABA (i.e., decreased the EC50) and increases the maximal efficacy with which GABA stimulated Cl- transport (i.e., increased the Emax). [3]
In Vivo
In vivo

Progesterone blocks the beneficial effect of estrogen on Abeta accumulation but not on behavioral performance in female 3xTg-AD mice. Progesterone significantly reduces tau hyperphosphorylation when administered both alone and in combination with estrogen. [4] Progesterone-treated rats are less impaired on a Morris water maze spatial navigation task than rats treated with the oil vehicle. Progesterone-treated rats also show less neuronal degeneration 21 days after injury in the medial dorsal thalamic nucleus, a structure that has reciprocal connections with the contused area. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06173869 Not yet recruiting Infertility Female Ferring Pharmaceuticals April 1 2024 Phase 3
NCT06193135 Not yet recruiting Infertility Female|Reproductive Sterility Instituto Valenciano de Infertilidad IVI VALENCIA March 1 2024 Not Applicable
NCT06155708 Not yet recruiting PCOS McGill University January 2024 Not Applicable
NCT04939064 Recruiting Normal Endometrial Physiology Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)|National Institutes of Health Clinical Center (CC) December 10 2023 --

Chemical lnformation & Solubility

Molecular Weight 314.46 Formula

C21H30O2
CAS No. 57-83-0 SDF Download Progesterone SDF
Smiles CC(=O)C1CCC2C1(CCC3C2CCC4=CC(=O)CCC34C)C
Storage (From the date of receipt)

In vitro
Batch:

Ethanol : 31.5 mg/mL

DMSO : 22 mg/mL ( (69.96 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Progesterone | Progesterone ic50 | Progesterone price | Progesterone cost | Progesterone solubility dmso | Progesterone purchase | Progesterone manufacturer | Progesterone research buy | Progesterone order | Progesterone mouse | Progesterone chemical structure | Progesterone mw | Progesterone molecular weight | Progesterone datasheet | Progesterone supplier | Progesterone in vitro | Progesterone cell line | Progesterone concentration | Progesterone nmr