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TAME

Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.

TAME Chemical Structure

TAME Chemical Structure

CAS: 901-47-3

Selleck's TAME has been cited by 11 Publications

1 Customer Review

Purity & Quality Control

Batch: S222501 DMSO] 69 mg/mL] false] Water] 69 mg/mL] false] Ethanol] 3 mg/mL] false Purity: 99.96%
99.96

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Biological Activity

Description Tosyl-L-Arginine Methyl Ester (TAME) is an APC inhibitor.
Targets
APC [1]
In vitro
In vitro TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. [1] TAME is hydrolysed by trypsin with Km of 0.328 mM. [2] TAME accelerates the ATP hydrolysis process about 12-fold. [3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. [4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. [5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M. [6]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05651620 Recruiting Calorie Restriction Tufts University|Pennington Biomedical Research Center|Washington University School of Medicine|Duke University|National Institute on Aging (NIA) March 21 2023 --

Chemical lnformation & Solubility

Molecular Weight 342.41 Formula

C14H22N4O4S
CAS No. 901-47-3 SDF Download TAME SDF
Smiles CC1=CC=C(C=C1)S(=O)(=O)NC(CCCN=C(N)N)C(=O)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 69 mg/mL ( (201.51 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 69 mg/mL

Ethanol : 3 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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