Home Apoptosis Caspase inhibitor Ac-DEVD-CHO For research use only.

Ac-DEVD-CHO

Synonyms: Caspase-3 Inhibitor I, N-Ac-Asp-Glu-Val-Asp-CHO

Ac-DEVD-CHO (Caspase-3 Inhibitor I, N-Ac-Asp-Glu-Val-Asp-CHO) is a potent aldehyde inhibitor of Group II caspases with Ki values of 0.2 nM and 0.3 nM for for caspase-3 and caspase-7, respectively. Weak inhibition for caspase-2.

Ac-DEVD-CHO Chemical Structure

Ac-DEVD-CHO Chemical Structure

CAS: 169332-60-9

Selleck's Ac-DEVD-CHO has been cited by 29 Publications

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Purity & Quality Control

Batch: S790101 Water] 100 mg/mL] false] ] ] false] ] ] false Purity: 98.86%
98.86

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Biological Activity

Description Ac-DEVD-CHO (Caspase-3 Inhibitor I, N-Ac-Asp-Glu-Val-Asp-CHO) is a potent aldehyde inhibitor of Group II caspases with Ki values of 0.2 nM and 0.3 nM for for caspase-3 and caspase-7, respectively. Weak inhibition for caspase-2.
Targets
Caspase-3 [1]
(Cell-free assay)		 caspase-8 [1]
(Cell-free assay)		 caspase-7 [1]
(Cell-free assay)		 caspase-10 [1]
(Cell-free assay)		 Caspase-1 [1]
(Cell-free assay)		 Click to View More Targets
230 pM(Ki) 0.92 nM(Ki) 1.6 nM(Ki) 12 nM(Ki) 18 nM(Ki)
In vitro
In vitro Ac-DEVD-CHO is a potent inhibitor of caspase-3 (Ki = 230 pM). In contrast, caspase-2 cleaves the tetrapeptide substrate poorly and is only weakly inhibited by this aldehyde (Ki = 1.7 μM). Group III caspases are broadly inhibited by Ac-DEVD-CHO with Ki values ranging from 1 to 300 nM[1]. Inhibition of caspase-3 by Ac-DEVD-CHO in isolated working-heart rat model significantly improves post-ischemic contractile recovery of stunned myocardium, even when given after the onset of ischemia. The mechanism(s) of protection by Ac-DEVD-CHO appear to be independent of apoptosis. Troponin I cleavage was not inhibited by Ac-DEVD-CHO[2].
Cell Research Cell lines MEFs
Concentrations 20, 50 μM
Incubation Time 2 h
Method

Apaf-1−/− cells were treated with the indicated concentration of Ac-DEVD-CHO for 2 h prior to treatment with 4 μM CHX alone or with TNF for 6 h. LEHDase and DEVDase activities were standardized to the lactate dehydrogenase activity in the sample and are presented as percentages of the activity in TNF-plus-CHX-treated cells not treated with Ac-DEVD-CHO (0 μM).
In Vivo
In vivo Ac-DEVD-CHO administered at the time of MI results in a 61% reduction in activated caspase-3 expression in cardiomyocytes (p<0.05), and an 84% reduction in cardiomyocyte apoptosis in the young animals. However, in the aging mice, caspase inhibition had no effect on activated caspase-3 expression or cardiomyocyte apoptosis[4]. Ac-DEVD-CHO suppressed and/or delayed the progression of photoreceptor cell damage in rats and delays disease progression in rd gene-carring mice, which normally develop retinal degeneration early in life[2].
Animal Research Animal Models C57Bl6 mice
Dosages 3 mg/kg
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 502.47 Formula

C20H30N4O11
CAS No. 169332-60-9 SDF Download Ac-DEVD-CHO SDF
Smiles CC(C)C(C(=O)NC(CC(=O)O)C=O)NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C
Storage (From the date of receipt)

In vitro
Batch:

Water : 100 mg/mL


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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