Home Metabolism Carbonic Anhydrase inhibitor Acetazolamide For research use only.

Acetazolamide

Synonyms: Diamox

Acetazolamide (Diamox), a potent carbonic anhydrase (CA) inhibitor, is commonly used in clinical practice as an immediate and readily available option for acute reduction of intraocular pressure(IOP).

Acetazolamide Chemical Structure

Acetazolamide Chemical Structure

CAS: 59-66-5

Selleck's Acetazolamide has been cited by 4 publications

Purity & Quality Control

Batch: Purity: 99.98%
99.98

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Biological Activity

Description Acetazolamide (Diamox), a potent carbonic anhydrase (CA) inhibitor, is commonly used in clinical practice as an immediate and readily available option for acute reduction of intraocular pressure(IOP).
Targets
Carbonic anhydrase [4]
10 nM
In vitro
In vitro acetazolamide reduces invasiveness of cancer cells in vitro[3].
Cell Research Cell lines renal carcinoma cell lines(Caki-1, Caki-2, ACHN, and A-498)
Concentrations 10 μM
Incubation Time 48 h
Method

The invasion assay is performed in a Biocoat Matrigel invasion chamber. Cells (n = 1.8 × 105) are added to the chamber and cultured for 48 h in the presence or absence of acetazolamide, which was administered twice during the 48-h period: the first dose at the beginning of the assay and the second after 24 h. After growing for 48 h, noninvasive cells are removed from the upper surface of the membrane with a cotton swab. The invasive cells on the lower surface of the membrane are fixed with 4% (vol/vol) neutral-buffered formaldehyde, stained with toluidine blue, and counted in 20 separate areas with a Leitz Diaplan microscope.
Experimental Result Images Methods Biomarkers Images PMID
Growth inhibition assay Cell viability 28235409
In Vivo
In vivo Acetazolamide reduces inflammation-induced heat hyperalgesia, probably acting both peripherally and centrally[1]. Acetazolamide shows significant inhibitory effect on angiogenesis in CAM and endothelial cell proliferation[2]. Acetazolamide is clinically used to reduce intraocular pressure in glaucoma, to correct metabolic alkalosis, and to manage cerebral edema. Its treatment may also be beneficial as an adjunct to cancer chemotherapy, because it can produce additive tumor growth delays with anticancer drugs in vivo[3].
Animal Research Animal Models Male Sprague-Dawley rats
Dosages 10, 100, 200 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06039475 Recruiting Acute Mountain Sickness|High Altitude Pulmonary Edema|High Altitude Cerebral Edema B.P. Koirala Institute of Health Sciences July 1 2023 --
NCT06114537 Recruiting Retinoschisis Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA) January 20 2023 Phase 2
NCT04679389 Active not recruiting Pmm2-CDG|CDG1A Mayo Clinic|Seattle Children''s Hospital|Children''s Hospital of Philadelphia March 17 2021 Phase 2|Phase 3
NCT03720288 Unknown status Heart Failure|Acute Heart Failure University of Sao Paulo General Hospital October 1 2018 Phase 3

Chemical lnformation & Solubility

Molecular Weight 222.25 Formula

C4H6N4O3S2
CAS No. 59-66-5 SDF Download Acetazolamide SDF
Smiles CC(=O)NC1=NN=C(S1)S(=O)(=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 44 mg/mL ( (197.97 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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