Home Metabolism Peroxidases chemical Alkyne tyramide For research use only.

Alkyne tyramide

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe, substantially improves APEX-labeling efficiency in intact yeast cells, also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins.

Alkyne tyramide Chemical Structure

Alkyne tyramide Chemical Structure

CAS: 1694495-59-4

Purity & Quality Control

Batch: E047101 DMSO] 43 mg/mL] false] Ethanol] 43 mg/mL] false] Water] 4 mg/mL] false Purity: 99.71%
99.71

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Biological Activity

Description Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe, substantially improves APEX-labeling efficiency in intact yeast cells, also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins.
Targets
APEX2 [1]

Chemical lnformation & Solubility

Molecular Weight 217.26 Formula

C13H15NO2
CAS No. 1694495-59-4 SDF --
Smiles OC1=CC=C(CCNC(=O)CCC#C)C=C1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 43 mg/mL ( (197.91 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 43 mg/mL

Water : 4 mg/mL


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In vivo
Batch:

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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