Home Metabolism DHFR inhibitor Aminopterin For research use only.

Aminopterin

Synonyms: 4-amino Folic Acid, 4-amino PGA, 4-Aminofolic acid, 4-Aminopteroylglutamic acid, Aminopteroylglutamic acid, APGA, NSC 739

Aminopterin (4-amino Folic Acid, 4-amino PGA, 4-Aminofolic acid, 4-Aminopteroylglutamic acid, Aminopteroylglutamic acid, APGA, NSC 739), a folic acid antagonist, is a competitive inhibitor of dihydrofolate reductase (DHFR) with Ki of 3.7 pM.

Aminopterin Chemical Structure

Aminopterin Chemical Structure

CAS: 54-62-6

Selleck's Aminopterin has been cited by 1 publication

Purity & Quality Control

Batch: E100301 DMSO] 88 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 98.83%
98.83

Other DHFR Products

Related compound libraries

Choose Selective DHFR Inhibitors

Biological Activity

Description Aminopterin (4-amino Folic Acid, 4-amino PGA, 4-Aminofolic acid, 4-Aminopteroylglutamic acid, Aminopteroylglutamic acid, APGA, NSC 739), a folic acid antagonist, is a competitive inhibitor of dihydrofolate reductase (DHFR) with Ki of 3.7 pM.
Targets
folic acid [1] DHFR [2]
(Cell-free assay)
3.7 pM(Ki)
In vitro
In vitro

In lymphoma celllines and several acute lymphoblastic leukaemia (ALL) cell lines (including COG-LL-356, NALM-6 and CCRF-CEM), the intensity of the cell kill effect, defined as maximal inhibition at the concentrations tested, is greater with Aminopterin than Methotrexate.[3]
Cell Research Cell lines lymphoma celllines and several ALL cell lines (including COG-LL-356, NALM-6 and CCRF-CEM)
Concentrations 1 nM - 10 μM
Incubation Time 72 h
Method

The concentrations of Aminopterin tested are 1 × 10-9 to 1 × 10-5 mol/l in threefold changes (1 × 10-9, 3 × 10-9, 1 × 10-8, 3 × 10-8, 1 × 10-7, 3 × 10-7, 1 × 10-6, 3 × 10-6, 1 × 10-5 mol/l). Cells are incubated with vehicle control or drugs for 72 h.
In Vivo
In vivo

In NOD/SCID mice, Aminopterin significantly delays the progression of ALL-3, ALL-16 and ALL-19, but not ALL-8 xenografts.[3]
Animal Research Animal Models NOD/SCID mice
Dosages 0.5 mg/kg, 0.25 mg/kg
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 440.41 Formula

C19H20N8O5
CAS No. 54-62-6 SDF --
Smiles NC1=NC(=C2N=C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)C=NC2=N1)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 88 mg/mL ( (199.81 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Aminopterin | Aminopterin supplier | purchase Aminopterin | Aminopterin cost | Aminopterin manufacturer | order Aminopterin | Aminopterin distributor