Home Epigenetics Histone Acetyltransferase inhibitor - Epigenetic Reader Domain inhibitor Anacardic Acid For research use only.

Anacardic Acid

Synonyms: 6-pentadecylsalicylic Acid

Anacardic Acid (6-pentadecylsalicylic Acid) is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.

Anacardic Acid Chemical Structure

Anacardic Acid Chemical Structure

CAS: 16611-84-0

Selleck's Anacardic Acid has been cited by 20 Publications

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Purity & Quality Control

Batch: Purity: 99.72%
99.72

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hi5 cells Function assay Non-competitive inhibition of human recombinant His-GST tagged VCP expressed in baculovirus infected Hi5 cells 23245311
HCT116 cells Cytotoxicity assay 72 h Cytotoxicity against human HCT116 cells after 72 hrs by luciferase reporter gene assay, IC50=0.38 μM 23245311
Hi5 cells Function assay Inhibition of human recombinant His-GST tagged VCP expressed in baculovirus infected Hi5 cells assessed as ADP formation incubated for 20 mins proir to substrate addition measured after 90 mins by NADH coupled assay, IC50=0.024 μM 23245311
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Biological Activity

Description Anacardic Acid (6-pentadecylsalicylic Acid) is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.
Targets
Tip60 [2]
(Cell-free assay)		 PCAF [1]
(Cell-free assay)		 p300/CBP [1]
(Cell-free assay)
5 μM 8.5 μM
In vitro
In vitro Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases[4]. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation[2]. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions[4].
Kinase Assay HAT and HDAC Activity Assays
After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.
Cell Research Cell lines HeLa and 293T cells, SQ20B and SCC35 squamous cell carcinoma cell lines
Concentrations 0-100 μM
Incubation Time 40 min
Method HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.
Experimental Result Images Methods Biomarkers Images PMID
Growth inhibition assay Cell viability 30210681
Western blot GRP78 / Hsp70 / CDK4 E-cadherin / Vimentin / MMP-9 ErbB2 30210681
In Vivo
In vivo Anacardic acid attenuates over-expression of Gata4, α-MHC and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of P300, PCAF to the promoter of Gata4[3].
Animal Research Animal Models 9 to 11 week old Kunming mice
Dosages 0, 1.25, 2.5, 5, 10 mg/kg
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 348.52 Formula

C22H36O3
CAS No. 16611-84-0 SDF Download Anacardic Acid SDF
Smiles CCCCCCCCCCCCCCCC1=C(C(=CC=C1)O)C(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 69 mg/mL ( (197.98 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 69 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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