Home Cytoskeletal Signaling HSP (HSP90) inhibitor - Apoptosis related activator Apoptozole For research use only.

Apoptozole

Synonyms: Apoptosis Activator VII

Apoptozole (Apoptosis Activator VII) is an inhibitor of heat shock protein 70(HSP70) and Hsc70 with dissociation constants Kd of 0.14 μM and 0.21 μM, respectively. It induces caspase-dependent apoptosis.

Apoptozole Chemical Structure

Apoptozole Chemical Structure

CAS: 1054543-47-3

Selleck's Apoptozole has been cited by 7 Publications

1 Customer Review

Purity & Quality Control

Batch: S836501 DMSO] 100 mg/mL] false] Ethanol] 25 mg/mL] false] Water] Insoluble] false Purity: 99.84%
99.84

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Biological Activity

Description Apoptozole (Apoptosis Activator VII) is an inhibitor of heat shock protein 70(HSP70) and Hsc70 with dissociation constants Kd of 0.14 μM and 0.21 μM, respectively. It induces caspase-dependent apoptosis.
Targets
HSP70 [1]
(Cell-free assay)		 HSC70 [1]
(Cell-free assay)
0.14 μM(Kd) 0.21 μM(Kd)
In vitro
In vitro Apoptozole induces caspase dependent apoptosis by blocking interaction of HSP70 with APAF-1, without affecting interactions of HSP70 with ASK1, JNK, BAX, and AIF[2]. However, apoptozole may form aggregates under aqueous conditions that could interact with HSP70 proteins in a non-specific manner, potentially leading to false positives and inconsistent data[3].
Cell Research Cell lines A549, lung adenocarcinoma epithelial cells; HeLa, cervical cancer cells; MDA-MB-231, breast cancer cells; HepG2, liver cancer cells
Concentrations 0-15 μM
Incubation Time 18 h
Method Several cancer cell lines (A549, lung adenocarcinoma epithelial cells; HeLa, cervical cancer cells; MDA-MB-231, breast cancer cells; HepG2, liver cancer cells) are treated with various concentrations (0-15 μM) of Az or compound 7 as a negative control for 18 hr. Cell viabilities are then determined using an MTT assay.
In Vivo
In vivo Apoptozole greatly retards tumor growth in mice xenografted with cancer cells without affecting mouse viability. The elimination half-life time (T1/2) of Az in blood is found to be significantly longer than that of Dox (8.04 versus 1.60 hr) and the time needed to reach a maximum concentration (Tmax) of Az is shorter than that of Dox (1.00 versus 4.00 hr)[2].
Animal Research Animal Models Male nude mice
Dosages 10 mg/kg
Administration i.p.

Chemical lnformation & Solubility

Molecular Weight 625.56 Formula

C33H25F6N3O3
CAS No. 1054543-47-3 SDF Download Apoptozole SDF
Smiles COC1=CC=C(C=C1)C2=C(N(C(=N2)C3=CC(=CC(=C3)C(F)(F)F)C(F)(F)F)CC4=CC=C(C=C4)C(=O)N)C5=CC=C(C=C5)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (159.85 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 25 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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