Home Apoptosis TRADD inhibitor Apostatin-1 (Apt-1) For research use only.

Apostatin-1 (Apt-1)

Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.

Apostatin-1 (Apt-1) Chemical Structure

Apostatin-1 (Apt-1) Chemical Structure

CAS: 2559703-06-7

Purity & Quality Control

Batch: S970501 DMSO] 69 mg/mL] false] Ethanol] 9 mg/mL] false] Water] Insoluble] false Purity: 99.56%
99.56

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Biological Activity

Description Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.
Targets
TRADD [1]
In vitro
In vitro

Apostatin-1 (Apt-1) is a TRADD inhibitor by combining with the pocket on the N-terminal TRAF2 binding domain of TRADD (TRADD-N) to modulate the ubiquitination of RIPK1 and beclin 1. Apostatin-1 effectively inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) in vitro.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Cell Research Cell lines Jurkat (ATCC TIB-152), MEF (CF-1) (ATCC SCRC-1040)
Concentrations 10 µM
Incubation Time 24 h for Jurkat cells; 8 h for mouse embryonic fibroblasts (MEFs)
Method

Jurkat cells were treated with Velcade (50 nM) and  Apt-1 (10 μM) for 24 h and cell viability was measured; In MEFs were treated with mTNF (1 ng/mL) and 5Z-7- Oxozeaenol (0.5 μM) to achieve RIPK1-dependent apoptosis (RDA),  added Apt-1 at different concentrations for 8 h and cell survival was measured.

Chemical lnformation & Solubility

Molecular Weight 345.50 Formula

C19H27N3OS
CAS No. 2559703-06-7 SDF --
Smiles CN1CCN=C1SC(C(=O)NC2CCCCCC2)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 69 mg/mL ( (199.71 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 9 mg/mL

Water : Insoluble


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In vivo
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