Home Metabolism SHIP inhibitor AS1949490 For research use only.

AS1949490

AS1949490, a competitive inhibitor of 5'-lipid phosphatase SHIP2 with IC50 values of 0.62 µM and 0.34 µM for human (Ki value is 0.44 µM) and mouse respectively, promotes protein kinase C-dependent stabilization of brain-derived neurotrophic factor mRNA in cultured cortical neurons.

AS1949490 Chemical Structure

AS1949490 Chemical Structure

CAS: 1203680-76-5

Purity & Quality Control

Batch: E040001 DMSO] 74 mg/mL] false] Ethanol] 37 mg/mL] false] Water] Insoluble] false Purity: 99.86%
99.86

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Biological Activity

Description AS1949490, a competitive inhibitor of 5'-lipid phosphatase SHIP2 with IC50 values of 0.62 µM and 0.34 µM for human (Ki value is 0.44 µM) and mouse respectively, promotes protein kinase C-dependent stabilization of brain-derived neurotrophic factor mRNA in cultured cortical neurons.
Targets
Human SHIP2 [1] Human SHIP2 [1] Mouse SHIP2 [1]
0.44 μM(Ki) 0.62 μM 0.62 μM
In vitro
In vitro

AS1949490 increases the phosphorylation of Akt, glucose consumption and glucose uptake in L6 myotubes. In FAO hepatocytes, AS1949490 suppresses gluconeogenesis.[1]
Cell Research Cell lines L6 myotubes, FAO hepatocytes
Concentrations 1-16 µM
Incubation Time 15 min, 24 h, 48 h
Method

L6 myotubes are incubated for 15 min both with and without AS1949490 treatment before being stimulated with insulin at indicated concentration and 1 nM or fetal bovine serum at indicated concentrations for 10 min. Protein lysates from L6 myotubes are immunoblotted with anti-phosphorylated Akt antibody or anti-Akt antibody. FAO cells are treated with AS19494940 and insulin for 24 h. The glucose concentration in the medium is measured after incubation for 6 h in gluconeogenesis buffer.
In Vivo
In vivo

Acute administration of AS1949490 inhibites the expression of gluconeogenic genes in the livers of normal mice. Chronic treatment of diabetic db/db mice with AS1949490 significantly loared the plasma glucose level and improved glucose intolerance.[1]

Chemical lnformation & Solubility

Molecular Weight 371.88 Formula

C20H18ClNO2S
CAS No. 1203680-76-5 SDF --
Smiles CC(NC(=O)C1=C(OCC2=CC=C(Cl)C=C2)C=CS1)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 74 mg/mL ( (198.98 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 37 mg/mL

Water : Insoluble


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In vivo
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