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ATP disodium

Name: ATP disodium
Brand: Selleck Chemicals
SKU: S1985
Rating: 5 (17 reviews)

Synonyms: Adenosine-Triphosphate disodium, Adenosine 5'-triphosphate

Catalog No.S1985 Purity: 99.79%

ATP disodium is a disodium salt form of adenosine-triphosphate which is a multifunctional nucleoside triphosphate.

ATP disodium Chemical Structure

CAS: 987-65-5

Selleck's ATP disodium has been cited by 17 publications

Purity & Quality Control

Batch: Purity: 99.79%

99.79

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Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor Y114A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.0301μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged wild type 4 human P2Y2 receptor expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.0631μM.	19419204
1321N1	Function assay			Agonist activity by measuring inositol phosphate accumulation in 1321N1 human astrocytoma cells stably expressing human P2Y purinoceptor 2, EC50=0.085μM.	11985476
1321N1	Function assay			Agonist activity at human P2Y2 receptor expressed in human 1321N1 cells coexpressing phospholipase C-activating G protein assessed as inositol phosphate production by scintillation proximity assay, EC50=0.085μM.	19419868
1321N1	Function assay			Agonist activity at human recombinant P2Y2 receptor expressed in 1321N1 cells assessed as stimulation of phospholipase C, EC50=0.085μM.	17302398
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged wild type 3 human P2Y2 receptor expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.0958μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R194H mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.111μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor Y198A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.12μM.	19419204
MDCK	Function assay	10 uM		Induction of intracellular calcium level in MDCK cells assessed as net peak value at 10 uM by Fura-2 in calcium free medium, Activity=0.132μM.	23163425
1321N1	Function assay		30 mins	Agonist activity at GFP tagged-human P2Y1 receptor expressed in human 1321N1 cells assessed as elevation in calcium level after 30 mins by fluorescence spectrophotometric analysis, EC50=0.15μM.	21090681
NG108-15	Function assay			Agonist activity at P2Y2 receptor in NG108-15 cells (unknown origin), EC50=0.18μM.	29254895
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R177A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.183μM.	19419204
HEK293	Function assay			Agonist activity at P2Y1 receptor expressed in human HEK293 cells, EC50=0.2μM.	20175517
HEK293	Function assay			Agonist activity at rat P2Y1R expressed in HEK293 cells assessed as release of intracellular calcium by fluorescence based assay, EC50=0.2μM.	22873688
CF/T43	Function assay			Agonist activity at P2Y2 receptor in human CF/T43 cells, EC50=0.2μM.	29254895
BEA	Function assay			Agonist activity at P2Y2 receptor in BEA cells (unknown origin), EC50=0.2μM.	29254895
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R180A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.237μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R177A_R180A double mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.239μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor Y118A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.299μM.	19419204
HEK 293	Function assay			Concentration required for calcium mobilization at rat purinergic 2Y1 receptor expressed in HEK 293 cells, EC50=0.59μM.	15317453
1321N1	Function assay			Antagonistic activity at human recombinant P2Y4 receptor expressed in 1321N1 cells, Kb=0.708μM.	17302398
1321N1	Function assay			Agonist activity at human GFP-tagged P2Y1R transfected in human 1321N1 cells assessed as induction of intracellular calcium mobilization by fluorescence assay, EC50=0.85μM.	23751098
1321N1	Function assay		30 mins	Agonist activity at human P2Y1 receptor expressed in human 1321N1 cells assessed as increase of intracellular calcium level after 30 mins using fura-2 AM by fluorescence assay, EC50=0.85μM.	24846781
1321N1	Function assay			Agonist activity at GFP-tagged human P2Y1R transfected in human 1321N1 cells assessed as increase in intracellular Ca2+ level by fura 2/AM probe-based fluorescence assay, EC50=0.85μM.	26447940
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor C278S mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=1μM.	19419204
1321N1	Function assay		30 mins	Antagonist activity at human P2X4 receptor expressed in 1321N1 cells assessed as inhibition of ATP-induced cytosolic calcium influx compound preincubated for 30 mins before ATP treatment by Fluo-4 AM fluorescence method, EC50=1μM.	23075067
Sf9	Function assay			Binding affinity to wild type 6XHis-tagged human JAK2 JH2 domain (513 to 827 residues) expressed in baculovirus infected Sf9 cells by MANT-ATP binding assay, Kd=1.3μM.	32329617
Sf9	Function assay			Binding affinity to 6XHis-tagged human JAK2 JH2 domain V617F mutant (513 to 827 residues) expressed in baculovirus infected Sf9 cells by MANT-ATP binding assay, Kd=1.3μM.	32329617
1321N1	Function assay			Accumulation of inositol phosphate in 1321N1 astrocytoma cells expressing human P2Y1 purinoceptor, EC50=1.5μM.	11985476
Sf9	Function assay			Binding affinity to recombinant human biotinylated N-terminal GST-tagged autophosphorylated TAK1 (1 to 303 residues) fused with TAB1 (437 to 504 residues) expressed in baculovirus infected sf9 cells by SPR assay, Kd=2.8μM.	28109791
1321N1	Function assay		30 mins	Agonist activity at GFP tagged-human P2Y11 receptor expressed in human 1321N1 cells assessed as elevation in calcium level after 30 mins by fluorescence spectrophotometric analysis, EC50=3.3μM.	21090681
1321N1	Function assay			Agonist activity at human GFP-tagged P2Y11R transfected in human 1321N1 cells assessed as induction of intracellular calcium mobilization by fluorescence assay, EC50=6.7μM.	23751098
1321N1	Function assay			Agonist activity at GFP-tagged human P2Y11R transfected in human 1321N1 cells assessed as increase in intracellular Ca2+ level by fura 2/AM probe-based fluorescence assay, EC50=6.7μM.	26447940
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R272A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=11.7μM.	19419204
1321N1	Function assay			Agonist activity by measuring inositol phosphate accumulation in 1321N1 human astrocytoma cells stably expressing human P2Y purinoceptor 11, EC50=17.3μM.	11985476
1321N1	Function assay			Antagonistic activity at human recombinant P2Y4 receptor expressed in 1321N1 cells	17302398
MDCK	Function assay	10 uM		Induction of intracellular calcium level in MDCK cells at 10 uM by Fura-2 in calcium free medium in presence of 10 uM U73343 inactive analogue of U73122 phospholipase C inhibitor	23163425
THLP1	Function assay			Agonist activity at NLRP3 in human THLP1-null cells assessed as induction of LPS-induced IL-1beta secretion by HEK-Blue reporter cell based assay	28829599
HEK293	Function assay	1 uM		Agonist activity at ALX/FPR2 (unknown origin) expressed in HEK293 cells co-expressing Galphaq assessed as induction of intracellular calcium accumulation at 1 uM by Fluo-4 dye based fluorescence spectrophotometric assay	30419493
HEK293	Function assay	1 uM		Induction of intracellular calcium accumulation in HEK293 cells at 1 uM by Fluo-4 dye based fluorescence spectrophotometric assay	30419493
BL21(DE3)	Function assay			Activity of Bacillus subtilis N-terminal His-tagged ANT(6) expressed in Escherichia coli BL21(DE3) cells assessed as adenylated product by Michaelis-Menten kinetics	ChEMBL
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Biological Activity

Description	ATP disodium is a disodium salt form of adenosine-triphosphate which is a multifunctional nucleoside triphosphate.

In vitro
In vitro	ATP release and autocrine feedback through P2Y2 and A3 receptors provide signal amplification, controlling gradient sensing and migration of neutrophils. ^[1] ATP results in production of reactive oxygen species (ROS), which stimulates the phosphatidylinositol 3-kinase (PI3K) pathway and subsequent Akt and ERK1/2 activation. ATP-dependent ROS production and PI3K activation also stimulate transcription of genes required for an oxidative stress response. ATP-mediated ROS-dependent PI3K is required for activation of caspase-1 and secretion of IL-1beta and IL-18. ^[2] ATP potently stimulates TNF-alpha release, resulting from TNF-alpha mRNA expression in rat cultured brain microglia. ATP-induced TNF-alpha release is Ca(2+)-dependent, and a sustained Ca(2+) influx correlated with the TNF-alpha release in ATP-stimulated microglia. ATP-induced TNF-alpha release is inhibited by PD 098059, an inhibitor of extracellular signal-regulated protein kinase (ERK) kinase 1 (MEK1), which activates ERK, and also by SB 203580, an inhibitor of p38 mitogen-activated protein kinase. ATP rapidly activates both ERK and p38 even in the absence of extracellular Ca(2+). ^[3] ATP-induced cytotoxicity is mediated by classical alterations of apoptosis, including membrane blebbing, nuclear condensation, and DNA fragmentation. ATP but not other nucleotides lead to the potent and selective activation of NF-κB in microglial cells through a P2Z receptor-mediated pathway. ^[4]

In Vivo
In vivo	ATP regulates microglial branch dynamics in the intact mice brain, and its release from the damaged tissue and surrounding astrocytes mediates a rapid microglial response towards injury. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT04760860	Not yet recruiting	Dementia With Lewy Bodies	Qiang Zhang\|University of Iowa	October 2024	Phase 1\|Phase 2
NCT05590195	Not yet recruiting	Bacterial Vaginosis\|Bacterial Infections\|Bacterial Vaginosis \| Vaginal \| Microbiology\|Vaginal Infection	Jeremy Burton\|Deerland Enzymes\|Lawson Health Research Institute\|St. Joseph''s Health Care London	May 1 2024	Phase 3
NCT06167512	Not yet recruiting	Multi-organ Failure After Severe Trauma	Assistance Publique - Hôpitaux de Paris	January 1 2024	--
NCT05938283	Not yet recruiting	Implantable Defibrillator User\|Hypertrophic Cardiomyopathy\|Implantable Cardioverter Ventricular Lead Dysfunction or Complication\|Ventricular Arrythmia	Barts & The London NHS Trust\|Boston Scientific Corporation\|Queen Mary University of London	January 1 2024	Not Applicable

References

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Chemical lnformation & Solubility

Molecular Weight	551.14	Formula	C₁₀H₁₄N₅O₁₃P₃.2Na
CAS No.	987-65-5	SDF	Download ATP disodium SDF
Smiles	C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)([O-])OP(=O)(O)[O-])O)O)N.[Na+].[Na+]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble

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In vivo
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Tech Support

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Handling Instructions

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