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Autophinib

Autophinib is a potent autophagy inhibitor with a novel chemotype with IC50 values of 90 and 40 nM for autophagy in starvation induced autophagy assay and rapamycin induced autophagy assay. The IC50 value for Vps34 is 19 nM in vitro.

Autophinib Chemical Structure

Autophinib Chemical Structure

CAS: 1644443-47-9

Selleck's Autophinib has been cited by 8 publications

Purity & Quality Control

Batch: S859601 DMSO] 5 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.60%
99.60

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Biological Activity

Description Autophinib is a potent autophagy inhibitor with a novel chemotype with IC50 values of 90 and 40 nM for autophagy in starvation induced autophagy assay and rapamycin induced autophagy assay. The IC50 value for Vps34 is 19 nM in vitro.
Targets
Vps34 [1] Autophagy [1]
(Rapamycin induced)		 Autophagy [1]
(starvation-induced)
19 nM 40 nM 90 nM
In vitro
In vitro Autophinib inhibits the accumulation of autophagosomes under fed as well as starved conditions, but does not affect the number of autolysosomes. Thus it is not a late stage autophagy inhibitor but an inhibitor of autophagosome formation. Autophinib targets VPS34 but not other lipid kinases, mTOR and TBK1. Autophinib appears to be ATP-competitive, targets VPS34 in cell lysate and induces cellular phenotypes consistent with VPS34 inhibition[1].
Cell Research Cell lines HeLa cells
Concentrations 30 μM
Incubation Time 18 h
Method

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Chemical lnformation & Solubility

Molecular Weight 346.73 Formula

C14H11ClN6O3
CAS No. 1644443-47-9 SDF Download Autophinib SDF
Smiles CC1=CC(=NN1)NC2=CC(=NC(=N2)OC3=CC=C(C=C3)[N+](=O)[O-])Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 5 mg/mL ( (14.42 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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