Home DNA Damage/DNA Repair PARP inhibitor Benzamide For research use only.

Benzamide

Benzamide, a derivative of benzoic acid, is an inhibitor of poly(ADP-ribose) polymerase with an IC50 of 3.3 μM.

Benzamide Chemical Structure

Benzamide Chemical Structure

CAS: 55-21-0

Selleck's Benzamide has been cited by 3 publications

Purity & Quality Control

Batch: S471501 DMSO] 24 mg/mL] false] Ethanol] 24 mg/mL] false] Water] Insoluble] false Purity: 98.79%
98.79

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Biological Activity

Description Benzamide, a derivative of benzoic acid, is an inhibitor of poly(ADP-ribose) polymerase with an IC50 of 3.3 μM.
Targets
PARP [4]
(Cell-free assay)
3.3 μM
In vitro
In vitro Benzamide, an inhibitor of PARP is protective against excitatory amino acid-induced cell death in primary cultures of neurons derived from neonatal rat brain. In addition, benzamide has more recently been shown to partially prevent the loss of dopaminergic cells and the increase in reactive gliosis caused by METH in vitro in fetal rat mesencephalic cells in culture[1]. Benzamide prevents transformation in a cell cycle-specific manner, maximal prevention coinciding with early S phase, also characteristic of maximal susceptibility to transformation[2].
Cell Research Cell lines Primary human fibroblasts
Concentrations 1 mM
Incubation Time 10 hr
Method

Exposure to carcinogens and benzamide is done 10 hr after the release of the metabolically induced G1/S block and exposure lasts 10 hr, followed by three washings and refeeding with fresh media.
In Vivo
In vivo PARP inhibitor benzamide is neuroprotective in C57Bl/6N mice against different types of neurotoxicities and without affecting body temperature[2]. Benzamide treatment significantly decreases the iNOS expression and number of apoptotic neurons and thereby improves the neuronal survival and memory during GCI. Benzamide administration (160 mg/kg i.p.) does not induce hypothermia and reaches the CNS in 30 min in the concentration range of 0.09-0.64 mM, at which, it shows neuroprotection[3].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01161836 Completed Advanced Solid Tumors Sanofi July 2010 Phase 1

Chemical lnformation & Solubility

Molecular Weight 121.14 Formula

C7H7NO
CAS No. 55-21-0 SDF Download Benzamide SDF
Smiles C1=CC=C(C=C1)C(=O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 24 mg/mL ( (198.11 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 24 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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