Home Transmembrane Transporters Sodium Channel inhibitor Benzocaine For research use only.

Benzocaine

Synonyms: ethyl 4-aminobenzoate

Benzocaine(ethyl 4-aminobenzoate) is the ethyl ester of p-aminobenzoic acid (PABA), it is a local anesthetic commonly used as a topical pain reliever or in cough drops.

Benzocaine Chemical Structure

Benzocaine Chemical Structure

CAS: 94-09-7

Purity & Quality Control

Batch: S421001 DMSO] 33 mg/mL] false] Ethanol] 33 mg/mL] false] Water] Insoluble] false Purity: 99.93%
99.93

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Biological Activity

Description Benzocaine(ethyl 4-aminobenzoate) is the ethyl ester of p-aminobenzoic acid (PABA), it is a local anesthetic commonly used as a topical pain reliever or in cough drops.
Targets
Sodium channel [1]
In vitro
In vitro

Benzocaine blocks µ1 wild-type Na+ currents in a dose-dependent manner with IC50 of 0.8 mM in HEK293T cells. Benzocaine (1 mM) blocks about 55% of wild-type Na+ current but about 95% of µ1-N1584A mutant current. Benzocaine (1 mM) blocks about 55% of wild-type µ1 currents, but about 80% of µ1-I1575A mutant current. [1] Benzocaine results in a biphasic (protective/inductive) concentration-dependent hemolytic effect upon rat erythrocytes, with an effective Benzocaine:lipid molar ratio in the membrane for protection (RePROT), onset of hemolysis (ReSAT) and 100% membrane solubilization (ReSOL) of 1.0:1, 1.1:1 and 1.3:1, respectively. [2] Benzocaine and 4-hydroxybenzoate interact with the open and inactivated channels during repetitive pulses, but during the interpulse the complex dissociates too fast to accumulate sufficient use-dependent block of Na+ currents. [3] Benzocaine (500 μM) reduces the peak and steady-state currents and increases the amplitude of the inactivating component from 21.7% to 30.2% (n=7, P<0.05), so that benzocaine-induced block at the end of pulses to +60 mV averaged 30.9% (n=7). Benzocaine (500 μM) significantly accelerates the initial phase of deactivation (τf=27.2±2.6 ms, n=7, P<0.01), but does not modify the slow phase of tail current decline. Benzocaine binds with high affinity to an intracellular binding site to produce 'agonist' effects and to a low affinity subsite, which is also located in the inner mouth, to produce the blocking effects. Benzocaine and extracellular K(+) interact to modify the voltage-dependence of channel opening. [4]
In Vivo
In vivo

Benzocaine is absorbed rapidly and similarly through both viable and nonviable skin of the hairless guinea pig, the absorption of the two acidic compounds, benzoic acid and PABA, is greater through nonviable skin. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06165432 Completed Pain B.P. Koirala Institute of Health Sciences March 19 2021 Phase 4
NCT03116737 Completed Pain|Acute Otitis Media Lachlan Pharma Holdings January 3 2017 Phase 3
NCT02092454 Completed Pain|Acute Otitis Media Otic Therapy LLC September 2013 Phase 2

Chemical lnformation & Solubility

Molecular Weight 165.19 Formula

C9H11NO2
CAS No. 94-09-7 SDF Download Benzocaine SDF
Smiles CCOC(=O)C1=CC=C(C=C1)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 33 mg/mL ( (199.76 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 33 mg/mL

Water : Insoluble


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