Home Transmembrane Transporters ADC Cytotoxin inhibitor - ATPase inhibitor BHQ For research use only.

BHQ

Synonyms: 2,5-di-t-butyl-1,4-benzohydroquinone

BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).

BHQ Chemical Structure

BHQ Chemical Structure

CAS: 88-58-4

Selleck's BHQ has been cited by 2 publications

Purity & Quality Control

Batch: S362801 Ethanol] 40 mg/mL] false] DMSO] 10 mg/mL] false] Water] Insoluble] false Purity: 99.52%
99.52

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Biological Activity

Description BHQ (2,5-di-t-butyl-1,4-benzohydroquinone) is a potent and selective inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPase (SERCA).
Targets
SERCA [1]
66.7 μM
In vitro
In vitro 2,5-Di-t-butyl-1,4-benzohydroquinone (BHQ) has been described as a rather selective sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitor. However, BHQ is not very specific, as it hits other sites of action besides SERCA. BHQ is shown, in fact, to reduce passive Ca2+ leakage from internal stores of permeabilized A7r5 vascular smooth muscle cells and to inhibit plasma membrane Ca2+ influx in parotid acinar cells, rat thymic lymphocytes, and GH3 pituitary cells. BHQ affects both endothelial and smooth muscle functions in rat aorta rings in vitro. BHQ induces endothelium-dependent relaxation by stimulating the NO synthase pathway, via activation of Ca2+ influx through Ni2+-sensitive Ca2+ channels in the endothelial cells. BHQ exhibits pleiotypic effects on rings deprived of endothelium by inducing both a myotonic response, dependent on the activation of Ca2+ influx via a Ni2+-sensitive pathway, and a myolytic effect, possibly dependent on either the depletion of intracellular Ca2+ stores or antagonism of Ca2+ entry via L-type Ca2+ channels. BHQ is a lipophilic, membrane permeable molecule, capable of generating superoxide anion at both sides of the plasmalemma[1].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03791086 Recruiting Bronchiectasis Adult University of Dundee November 1 2019 --
NCT01302886 Completed Smoldering Multiple Myeloma Novartis Pharmaceuticals|Novartis May 2011 Phase 2
NCT00741377 Completed Multiple Myeloma Bone Disease Novartis Pharmaceuticals|Novartis January 2009 Phase 1

Chemical lnformation & Solubility

Molecular Weight 222.32 Formula

C14H22O2
CAS No. 88-58-4 SDF Download BHQ SDF
Smiles CC(C)(C)C1=CC(=C(C=C1O)C(C)(C)C)O
Storage (From the date of receipt)

In vitro
Batch:

Ethanol : 40 mg/mL

DMSO : 10 mg/mL ( (44.98 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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