Home Cell Cycle Microtubule Associated inhibitor Colchicine For research use only.

Colchicine

Synonyms: Colchineos, Colchisol, Colcin,NSC 757

Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.

Colchicine Chemical Structure

Colchicine Chemical Structure

CAS: 64-86-8

Selleck's Colchicine has been cited by 19 publications

Purity & Quality Control

Batch: Purity: 99.99%
99.99

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Biological Activity

Description Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.
In vitro
In vitro

Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.[1]
Cell Research Cell lines HeLa cells
Concentrations 5 μM
Incubation Time 2 h
Method

HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed.
In Vivo
In vivo

Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.[2]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05855746 Not yet recruiting Acute Myocarditis Assistance Publique - Hôpitaux de Paris|Hospices Civils de Lyon|Fonds de Dotation ACTION January 22 2024 Phase 3
NCT06020300 Recruiting STEMI National University of Malaysia July 28 2023 Phase 4
NCT05472337 Recruiting Acute Coronary Syndrome Pontificia Universidad Catolica de Chile|Fondo Nacional de Desarrollo Científico y Tecnológico Chile August 1 2022 Phase 2

Chemical lnformation & Solubility

Molecular Weight 399.44 Formula

C22H25NO6
CAS No. 64-86-8 SDF Download Colchicine SDF
Smiles CC(=O)NC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 80 mg/mL ( (200.28 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 80 mg/mL

Water : 79 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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