Home Transmembrane Transporters Chloride Channel inhibitor Dehydroandrographolide For research use only.

Dehydroandrographolide

Dehydroandrographolide, isolated from Andrographis paniculata (Burm. F.) Nees (Chuan-xin-lian), is a novel TMEM16A inhibitor and possesses multiple pharmacological activities, including anti-inflammation, anti-cancer, anti-bacterial, anti-virus and anti-hepatitis activity.

Dehydroandrographolide Chemical Structure

Dehydroandrographolide Chemical Structure

CAS: 134418-28-3

Purity & Quality Control

Batch: Purity: 99.99%
99.99

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Biological Activity

Description Dehydroandrographolide, isolated from Andrographis paniculata (Burm. F.) Nees (Chuan-xin-lian), is a novel TMEM16A inhibitor and possesses multiple pharmacological activities, including anti-inflammation, anti-cancer, anti-bacterial, anti-virus and anti-hepatitis activity.
Targets
TMEM16A [1]
In vitro
In vitro Dehydroandrographolide inhibits TMEM16A chloride currents in Fisher rat thyroid (FRT) cells that are transfected stably with human TMEM16A and in TMEM16A-overexpressed SW620 cells but does not alter cystic fibrosis transmembrane conductance regulator (CFTR) chloride currents. Further functional studies show that dehydroandrographolide suppresses the proliferation of SW620 cells in a dose- and time-dependent manner using MTT assays. Dehydroandrographolide significantly inhibits migration and invasion of SW620 cells as detected by wound-healing and transwell assays. Moreover, treatment of SW620 cells with dehydroandrographolide leads to a decrease in TMEM16A protein levels but has no effect on TMEM16A mRNA levels[1]. Dehydroandrographolide induces autophagy in human oral cancer cells by modulating p53 expression, activating JNK1/2, and inhibiting Akt and p38. Dehydroandrographolide is an iNOS inhibitor and an antiinflammatory and antiviral agent[2].
Cell Research Cell lines SW620 cells
Concentrations 5, 10, 20, 40 or 80 μM
Incubation Time 24 h
Method Cells (2 × 104 cells/ml) in the absence or presence of DP at different concentration are incubated for 24 h in 24-well tissue culture plates. At the end of the incubation period, the culture medium is removed and cells are washed with phosphate-buffered saline (PBS pH 7.4) and the cells are photographed under an inverted microscope at 100 × magnification.
In Vivo
In vivo Administration of dehydroandrographolide effectively suppresses the tumor formation in the oral carcinoma xenograft model in vivo[2].
Animal Research Animal Models 5–6 week male BALB/c nude mice
Dosages 40 or 100 mg/kg
Administration oral

Chemical lnformation & Solubility

Molecular Weight 332.43 Formula

C20H28O4
CAS No. 134418-28-3 SDF Download Dehydroandrographolide SDF
Smiles CC12CCC(C(C1CCC(=C)C2CC=C3C=COC3=O)(C)CO)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 66 mg/mL ( (198.53 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 33 mg/mL

Water : Insoluble


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In vivo
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