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Deoxycholic acid

Name: Deoxycholic acid
Brand: Selleck Chemicals
SKU: S4689
Rating: 5 (1 reviews)

Synonyms: Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic

Catalog No.S4689 Purity: 99.94%

Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

Deoxycholic acid Chemical Structure

CAS: 83-44-3

Selleck's Deoxycholic acid has been cited by 1 publication

Purity & Quality Control

Batch: S468901 DMSO] 78 mg/mL] false] Ethanol] 60 mg/mL] false] Water] Insoluble] false Purity: 99.94%

99.94

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Biological Activity

Description	Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

In vitro
In vitro	Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway^[1].
Cell Research	Cell lines	Primary rat hepatocytes
	Concentrations	25 to 200 μM
	Incubation Time	24 h
	Method	Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 10⁴ cells/cm². Cells are kept at 37 °C in a humidified atmosphere of 5% CO₂ for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining.

In Vivo
In vivo	miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage^[1].
Animal Research	Animal Models	Male Wistar rats
	Dosages	125 mg/kg
	Administration	p.o.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT02654496	Completed	Obesity	North Dakota State University	January 2016	--
NCT01865812	Completed	Primary Biliary Cirrhosis	Intercept Pharmaceuticals	December 3 2013	Phase 2
NCT01319916	Completed	Healthy	Kythera Biopharmaceuticals	December 2010	Phase 1

References

[1] Pedro M. Rodrigues, et al. Sci Rep. 2015, 5:17528.

Chemical lnformation & Solubility

Molecular Weight	392.57	Formula	C₂₄H₄₀O₄
CAS No.	83-44-3	SDF	Download Deoxycholic acid SDF
Smiles	CC(CCC(=O)O)C1CCC2C1(C(CC3C2CCC4C3(CCC(C4)O)C)O)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 78 mg/mL ( (198.69 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble

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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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